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  • Biomass  (1)
  • Brassicaceae  (1)
  • Chronic bronchitis Iron miners  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 1234-1241 
    ISSN: 1432-2242
    Schlagwort(e): Key words Diplotaxis ; Brassicaceae ; ISSR-PCR ; Genetic relationships ; Molecular taxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Inter-simple sequence repeat (ISSR) amplification was evaluated for its applicability as a genetic marker system to establish relationships among ten Diplotaxis species. ISSR amplification generated multiple banding profiles with the 12 primers from all DNA samples, with an average of 41.2 fragments per primer. This average was clearly higher for the 5´ triple-anchored primers than for other primers. The banding profiles were highly repeatable across separate PCR runs. DNA mixing procedures were found to be appropriate strategies to generate banding patterns representative of each species studied. Similarity values were calculated considering 494 ISSR bands, and a dendrogram was constructed based on the similarity matrix. The ten Diplotaxis species were clustered into two major groups. The first group consists of five species, Diplotaxis tenuifolia and Diplotaxis cretacea, and Diplotaxis muralis with their putative parents (D. tenuifolia and Diplotaxis viminea). In the second group three species are clustered that are closely related (Diplotaxis virgata, Diplotaxis catholica and Diplotaxis siettiana), in addition to Diplotaxis harra, and Diplotaxis erucoides, which has lowest similarity values with the rest of the species studied. The two groups defined in the present work may be concordant with the idea suggested by several authors of a biphyletic origin for Diplotaxis. The genetic relationships among the ten Diplotaxis species estimated by the polymorphism of ISSR markers are in agreement with those previously inferred by other morphological, biochemical and molecular data, indicating the reliability of the ISSR approach for this purpose.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-5036
    Schlagwort(e): Biodegradation ; Biomass ; Carbon ; Cornstalks ; Humus formation ; Wheat straw
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The effect of soil type and incubation period on the biodegradation, incorporation into biomass, and stabilization in humus of14C-labeled cornstalk and/or wheat straw lignin, polysaccharide, and protein fractions were followed for one year. After 6 months, 56–68%, 6–21%, 71–81%, 63–75%, and 56–68% from wheat straw and from the lignin, polysaccharide, and protein fraction of wheat straw had been lost as CO2, respectively. Loss of CO2 increased only slightly with further incubation. Greater amounts of CO2, especially during the early incubation stages, were evolved from neutral and alkaline soils (pH 7.0, 7.4, 7.8) than from acid soils (pH 5.0, 5.5). After one year, a major portion of the residual C from lignin was recovered in the humic acid fraction, relatively small amounts, 5 to 17%, were lost upon acid hydrolysis, and generally 〈1% was found present in the biomass. Lesser amounts of the polysaccharide and protein carbons were incorporated into the humic acid, 17–20% and 16–27%, respectively. Relatively greater amounts of the residual carbons of the polysaccharide and protein were incorporated into the biomass, 4.9–7.8% and 4.6–13.4%, respectively and higher percentages were lost upon acid hydrolysis, 56 to 81%. The results for the whole wheat straw were very similar to those of the protein fraction. Overall, more residual C was stabilized into humic acid in the acid soils than in the neutral soils.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    European journal of epidemiology 4 (1988), S. 39-44 
    ISSN: 1573-7284
    Schlagwort(e): Epidemiology ; Occupational pathology ; Chronic bronchitis Iron miners ; Protease inhibitor (Pi) phenotype
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Pi phenotype was determined and α1 protease inhibitor (α1Pi) was measured in 871 iron-ore miners examined twice at five years interval. A questionnaire on respiratory symptoms was administered and lung function tests: spirometry (VC, FEV1.0), measurements of the residual volume (RV/TLC) and of the CO diffusion (FuCO), were carried out by the same medical and technical team, using the same apparatus. There were no differences in age, lenght of employment or smoking habits among the three Pi Phenotype groups: M (90:03%), MS (7.1%), MZ (2.8%). The prevalence of clinical symptoms at the initial survey and the incidence of symptoms between the 2 surveys were not related to Pi Phenotype groups. However a significantly steeper decline of FEV1.0/VC was observed in the α1Pi partially deficient groups MS and MZ (−3.9%) compared with the non-deficient group (−1.8%). No significant difference was observed for VC, RV /TLC and FuCO. This finding suggests that an intermediate deficiency of α1Pi may be related to a slight but statistically significant impairment of a lung function in occupational conditions.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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