ISSN:
1432-2242
Keywords:
Key words Transgenic bean
;
Phaseolus vulgaris
;
Co-transformation
;
Biolistic
;
Inheritance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050259
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