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  • 1
    Electronic Resource
    Electronic Resource
    Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device (1993)
    Springer
    Plant cell reports 12 (1993), S. 483-490 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The parameters influencing transient expression of the betaglucuronidase gene in bean embryonic axes, cotyledons, apical meristems and leaves were evaluated after gene delivery with an electrical particle acceleration device. A calciumspermidine procedure for coating gold particles with DNA resulted in higher levels of GUS expression with lower concentrations of gold particles compared with a calcium phosphate procedure. The DNA concentration, distance between the discharge chamber and the retaining screen and the vacuum in the apparatus also influenced gene delivery. Sections prepared for light and electron microscopy showed the localisation, within target cells, of gold particles used to deliver the DNA. Immunolocalization of foreign gene expression within cells confirmed an even distribution of gene product throughout the cell cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236092
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 142-150 
    Details
    ISSN: 1432-2242
    Keywords: Key words Transgenic bean ; Phaseolus vulgaris ; Co-transformation ; Biolistic ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050259
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 142-150 
    Details
    ISSN: 1432-2242
    Keywords: Transgenic bean ; Phaseolus vulgaris ; Co-transformation ; Biolistic ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225739
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Partial resistance to anthracnose in Brazilian land races of dry beans shows race specificity (1988)
    Springer
    Euphytica 39 (1988), S. 167-174 
    Details
    ISSN: 1573-5060
    Keywords: Phaseolus vulgaris ; dry bean ; Colletotrichum lindemuthianum ; anthracnose ; horizontal resistance ; race-nonspecific resistance ; land race
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifty-four land races of dry beans (Phaseolus vulgaris), indigenous to areas of Brazil where anthracnose (caused by Colletotrichum lindemuthianum) is a common problem, were evaluated in field nurseries for partial resistance to race Brazilian 1 (B1) of C. lindemuthianum using symptom severity classes (SSC) from 0 to 6. Plants were selected if symptoms were present and the SSC was less than the 95% confidence interval of the mean SSC of the susceptible cultivar Carioca. S1 progeny from selected plants were evaluated in air-conditioned chambers for partial resistance to races B1, delta, and kappa of C. lindemuthianum. Of 246 S1 families evaluated, 145 families were partially resistant to one or two of the races [symptoms present, but S1 family mean significantly (p〈0.05) less than the mean of Carioca] and susceptible to the third. Six families were partially resistant to all three races. The remaining families were either susceptible or segregated for reaction to race B1. Partial resistance to C. lindemuthianum showed race specificity in the air-conditioned chambers and field nurseries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039870
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    Paper (German National Licenses)
    Fulltext
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