ISSN:
1432-2048
Keywords:
Coleus (cell cultures)
;
Hydroxycinnamic acid ester
;
Rosmarinic acid
;
Rosmarinic acid synthase (purification)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Rosmarinic acid synthase from cell cultures of Coleus blumei Benth. was purified to apparent homogeneity by fractionated ammonium sulfate precipitation (60–80% saturation), hydrophobic interaction chromatography, affinity chromatography and gel filtration. This purification procedure resulted in a 225-fold-enriched specific enzyme activity with a yield of 9%. The protein preparation was apparently pure according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis. The apparent molecular mass determined by gel filtration and SDS-PAGE was 77 kDa, indicating that rosmarinic acid synthase is a monomeric enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00240891
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