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  • 1
    Electronic Resource
    Electronic Resource
    Liquid chromatographic separation of monoamino analogues of dideoxyadenosine and 9-(dideoxy-β-D-lyxo-pentofuranosyl)adenine from adenine (1993)
    Springer
    Chromatographia 35 (1993), S. 451-454 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Adenine ; Monoamino analogues of dideoxyadenosine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A liquid chromatographic method that can separate each of the monoamino analogues of dideoxyadenosine and 9-(-dideoxy-β-D-lyxo-pentofuranosyl)adenine from their main degradation product, adenine, is described. The influence of the pH of the mobile phase, the type and concentration of the organic modifier and the concentration of the silanol masking agent and the buffer have been investigated. Several reversed phases were examined. Samples were finally analyzed on a 10 μm Spherisorb ODS1 column (250 mm×4.6 mm I.D.) using acetonitrile −0.2 M potassium phosphate buffer pH 6.0–0.2 M tetramethylammonium phosphate pH 6.0-water (5∶5∶0.5∶89.5, v/v) as the mobile phase. The interactions between the solute and the stationary phase are discussed in view of the basic properties of the compounds under study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02278601
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Interlaboratory study of analysis of phenoxymethylpenicillin by liquid chromatography (1998)
    Springer
    Chromatographia 47 (1998), S. 152-156 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Phenoxymethylpenicillin ; Collaborative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A liquid chromatography method for analysis of phenoxymethylpenicillin has been examined in a collaborative study involving 6 laboratories. The method comprised an isocratic part, which is used in the assay. When the isocratic part is combined with gradient elution, the method is suitable for purity control. Five samples of phenoxymethylpenicillin (potassium salts and acid) were analysed. The main component, the most important side product 4-hydroxyphenoxymethyl-penicillin and other impurities were determined. An analysis of variance proved the absence of consistent laboratory bias. Laboratory-sample interaction was not significant. Estimates of the repeatability and reproducibility of the method, expressed as standard deviations (SD) of the result of the determination of phenoxymethylpenicillin, were 0.50 and 0.63 respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02466574
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    High performance liquid chromatography of chlortetracycline and related substances on poly(styrenedivinylbenzene)copolymer (1990)
    Springer
    Chromatographia 30 (1990), S. 105-109 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlortetracycline and related substances ; Poly(styrene-divinylbenzene)copolymer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An isocratic high performance liquid chromatographic method has been developed for assay and purity control of chlortetracycline, which is separated from all related compounds. The stationary phase is poly(styrene-divinylbenzene)copolymer (PSDVB), which is heated at 60°C. The mobile phase is 2-methyl-2-propanol-1.0 M perchloric acid (5.0 ml)-water (up to 100 ml). The amount of alcohol modifier varies between 2.5 and 6.5% m/v depending upon the brand and porosity of PSDVB used. The flow rate is 1.0 ml/min and UV detection is at 254 nm. The total analysis time does not exceed 40 min. Although the method has been shown to be applicable with several brands of 100 Å PSDVB e.g. PLRP-S, PRP-1, RoGeL and TSK-Gel, the best separations were obtained on the wide pore material PLRP-S 100 Å.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02270458
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Liquid chromatographic separation of the anomers and isomers of 2′-deoxyuridine (1992)
    Springer
    Chromatographia 33 (1992), S. 571-574 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; 2′-Deoxyuridine ; Anomer separation ; Isomer separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A liquid chromatographic assay method was developed to separate 2′-deoxyuridine from its anomer and pentopyranosyl isomers. The influence of the pH of the mobile phase, the type of organic modifier and the column temperature on the chromatographic parameters was systematically investigated. Using a Hypersil C18 (5 μm) column at 10°C, the following mobile phase was found suitable: tetrahydrofuran −0.2 M potassium phosphate buffer pH 4.0-water (0.1∶5∶94.9, v/v).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262250
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Liquid chromatography of erythromycin A and related substances on poly(styrene-divinylbenzene) (1991)
    Springer
    Chromatographia 32 (1991), S. 162-166 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Erythromycin A and related substances ; Poly(styrene-divinylbenzene)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An isocratic liquid chromatography method for assay and purity control of erythromycin is presented. Erythromycin A is separated from all its potential impurities, except erythromycin D. The selectivity depends on the pore size of the poly(styrene-divinylbenzene) stationary phase. Wide pore PLRP-S 8 μm 1000 Å shows the best selectivity. The column is heated at 70°C. The mobile phase is acetonitrile-2-methyl-2-propanol-0.2 M potassium phosphate buffer pH 9.0-water (3:16.5:5:75.5). The flow rate is 2 ml/min. UV detection is performed at 215 nm. The total analysis time is about 30 min. The method was used to compare official standards.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02325020
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of analytical methods. Analysis of minocycline by liquid chromatography (1992)
    Springer
    Chromatographia 33 (1992), S. 560-566 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Official methods of analysis ; Method evaluation ; Minocycline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The liquid chromatographic methods laid out for the analysis of minocycline hydrochloride in the British Pharmacopoeia 1988, the United States Pharmacopeia XXII and the Pharmacopée Française 10 were examined. The major difference between these methods lies in the adjustment of the pH of the mobile phase. The analyst has to obtain the right pH of the mobile phase for his available column. This was found to be a less than easy task for the occasional analyst. Other disadvantages observed were poor solubility of the components of the mobile phase, reduced sensitivity due to UV absorbance by the mobile phase, rather high back pressure, the elution order for 9-minocycline depending on the brand of stationary phase, and above all, poor stability of the stationary phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262248
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Collaborative study of the analysis of chlortetracycline by liquid chromatography on octylsilyl silica gel (1993)
    Springer
    Chromatographia 37 (1993), S. 640-644 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlortetracycline ; Reversed-phase silical gel ; C8 bonded phase ; Collaborative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An established method for the analysis of chlortetracycline by liquid chromatography using octylsilylated silica gel as the stationary phase was examined in a multicentre study involving five laboratories and a total of six columns. Three chlortetracycline hydrochloride samples were analysed. The main component and the impurities were determined. An analysis of variance, treating each column as a different laboratory, showed an absence of consistent between-laboratory bias and the presence of a significant laboratory-sample interaction. The repeatability and the reproducibility of the method, expressed as relative standard deviations of the result of the determination of chlortetracycline hydrochloride, were calculated to be 0.9% and 1.1% respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02274116
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    Paper (German National Licenses)
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