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Tangential orientation and spatial order in dendrites of cat auditory cortex: A computer microscope study of Golgi-impregnated material (1979)

Glaser, E. M. ; Loos, H. ; Gissler, M.

Springer

Experimental brain research 36 (1979), S. 411-431

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ISSN: 1432-1106

Keywords: Auditory cortex ; Computer microscopy ; Dendrites ; Golgi ; Orientation

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary In the tangential plane (parallel to the pial surface) dendrites in the primary auditory cortex (A1) of cat were found to exhibit preferentially oriented growth. This was shown by means of a computer microscope study of Golgi-Cox stained neurons as seen in 100 μm and 300 μm thick tangential sections. Two techniques were used to represent the 3-dimensional structure of dendrites: the “dendritic stick” and the “dendritic trumpet”. The former dismembers a dendrite into its individual segments; the latter considers a dendrite as an entity and represents it by its centroid, its moments and the spatial dispersion of its branches. Both statistical and Fourier analyses of the data show that within the tangential plane there is a significant and consistent orientation of the dendritic sticks in a dorso-ventral direction which seems correlated with the cortical isofrequency contours observed in electrophysiological maps of the A1 region. The dendritic trumpet analyses also show a distinctly non-random vertical distribution of pyramidal cell basal dendrites but not of stellate cell dendrites.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00238513

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A model system for a fluorometric biosensor using permeabilized Zymomonas mobilis or enzymes with protein confined dinucleotides (1993)

Thordsen, O. ; Lee, S. J. ; Degelau, A. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 42 (1993), S. 387-393

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ISSN: 0006-3592

Keywords: biosensor ; glucose ; fructose ; gluconolactone ; sorbitol ; Zymomonas mobilis ; permeabilization ; NADPH ; fluorescence ; bioprocess monitoring ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Using permeabilized Zymomonas mobilis or glucose-fructose oxidoreductase isolated from this microorganism a model system for biosensors with a protein confined NADP(H) cofactor for the determination of glucose, fructose, gluconolactone, and sorbitol was developed. Either permeabilized microorganisms containing the oxidoreductase or the pure enzyme were confined via membrane separation in a small measuring chamber, that was integrated into a flow injection analysis system (FIA). The measuring principle was the monitoring of the NAD(P)H fluorescence, excited at 360 nm and measured at 450 nm. NADP(H), which is confined in the protein complex, was oxidized or reduced during the enzymatic reactions and the changes in the fluorescence intensity were related to the substrate concentration. The sensitivity of the system covered a range from 0.001 to 100 g/L of the analyte depending on substrate and operating conditions. The applicability of this model system for bioprocess monitoring was proved using samples from a Pseudomonas pseudoflava cultivation. © 1993 John Wiley & Sons, Inc.

Additional Material: 13 Ill.