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  • Ultrastructure  (2)
  • Cytoplasmic migration  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The taxonomic significance of the mitotic spindle and nucleus-associated organelle ofEntomophaga aulicae (1984)
    Springer
    Protoplasma 120 (1984), S. 84-90 
    Details
    ISSN: 1615-6102
    Keywords: Entomophaga aulicae ; Fungi ; Mitosis ; Nucleus associated organelle ; Taxonomy ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nuclei in protoplasts ofEntomophaga aulicae contain abundant condensed chromatin and a large central nucleolus. The metaphase spindle occupies a small eccentric area of the nucleus while the remainder of the nucleus is filled with condensed chromatin. Small portions of condensed chromatin are aligned along a broad metaphase plate and connected to the spindle poles by kinetochore microtubules. The nucleus associated organelle (NAO) is a solid barlike structure which lies at the spindle poles and is closely associated with the outer membrane of the nuclear envelope. Comparison of the nuclear characteristics ofE. aulicae with those of other members of theEntomophthorales supports the separation of theEntomophthoraceae from theBasidiobolaceae andAncylistaceae. Further comparison of details of nuclear division in theEntomophthoraceae, specifically NAO morphology, may be useful in helping to delineate evolutionary lines within the family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287620
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Observations on the ultrastructure of microtubules (1971)
    Springer
    Cell & tissue research 115 (1971), S. 388-395 
    Details
    ISSN: 1432-0878
    Keywords: Microtubule ; Ultrastructure ; Spindle ; Flagellum ; Subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After a specific time of glutaraldehyde-acrolein fixation, microtubule walls appear to be composed of single 6.5–7.5 nm diameter osmiophilic subunits. Variations in the duration of glutaraldehyde-acrolein and also glutaraldehyde-osmium fixation reveal a two layered wall containing osmiophilic subunits, 4.0–4.5 nm in diameter, arranged radially, in tandem. The double-layered wall is demonstrated by microdensitometer traces. These observations are discussed in relation to previously proposed models of microtubule substructure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324941
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    UV microirradiations elicit Ca2+-dependent apex-directed cytoplasmic contractions in hyphae (1992)
    Springer
    Protoplasma 170 (1992), S. 46-52 
    Details
    ISSN: 1615-6102
    Keywords: Cytoplasmic migration ; Tip growth ; Actin ; Calcium ; UV microirradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Polarized tip-ward cytoplasmic contractions were induced in hyphae ofSaprolegnia ferax with ultraviolet microirradiations. These unidirectional contractions were similar in appearance and ionic requirements to those previously induced in hyphae ofBasidiobolus magnus, suggesting that the observed inherent cytoplasmic polarity is a general phenomenon. During growth the cytoplasm is continually moving forward with respect to the lateral cell wall and plasma membrane in order to maintain its position in the tip. These contractions may be an exaggerated form of this cytoplasmic migration. F-actin was most concentrated in the contracted cytoplasm, implying that it may be involved in generating the contraction. Contractions were enhanced by external Ca2+ and by irradiating the tip region which is rich in Ca2+ sequestering organelles, suggesting that flooding of the cytoplasm with Ca2+ caused the contractions. H+ did not affect contraction frequency. Neither the change in cytoplasmic consistency that preceded contraction, the contraction itself, nor the F-actin damage induced were confined to the microirradiated zone. This is in keeping with irradiation-induced damage to a network under tension or a flux of diffusible ions causing the response. Thus Ca2+ may regulate actin-myosin interactions that generate cytoplasmic migration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01384456
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    Paper (German National Licenses)
    Fulltext
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