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  • DNA amplification  (2)
  • ATP-synthase  (1)
  • Allosteric model Binding Gating Neuronal nicotinic receptors Single channel  (1)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Bilayer thickness and enzymatic activity in the mitochondrial cytochrome c oxidase and ATPase complex
    Amsterdam : Elsevier
    FEBS Letters 144 (1982), S. 145-148 
    Details
    ISSN: 0014-5793
    Keywords: ATP-synthase ; Bilayer thickness ; Cytochrome oxidase ; Protein-lipid interaction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(82)80588-7
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Gating of α3β4 neuronal nicotinic receptor can be controlled by the loop M2-M3 of both α3 and β4 subunits (1999)
    Springer
    Pflügers Archiv 439 (1999), S. 86-92 
    Details
    ISSN: 1432-2013
    Keywords: Allosteric model Binding Gating Neuronal nicotinic receptors Single channel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Previous studies have shown that the gating mechanism of α3β4 neuronal nicotinic receptors is affected by a residue in the middle of the M2-M3 loop of the β4 subunit. We have extended the study of the same location to the α3 subunit. Bovine α3β4 receptors were mutated in position 268, substituting the residue present in wild-type receptors, i.e. leucine in α3 and asparagine in β4, for an aspartate. Wild-type and mutated α3 and β4 subunits were combined to form four different receptors. We have measured macroscopic currents in Xenopus oocytes elicited by nicotine, and related them to surface receptor expression measured with an epibatidine-binding essay. We also obtained single-channel recordings of the receptors to study their kinetic behaviour. The results were analysed in terms of an allosteric model with three states. We found that the effect of the mutation in the α3 subunit on the gating of the receptor was similar to the corresponding mutation in the β4 subunit. The effect when both subunits were mutated was additive, suggesting that the contribution of each subunit to the gating mechanism is independent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900143
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Nuclear DNA amplification in cultured cells of Oryza sativa L. (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 65-70 
    Details
    ISSN: 1432-2242
    Keywords: DNA amplification ; Cultured cells ; Dot hybridization ; Oryza sativa L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Highly repeated nuclear DNA sequences from suspension cultured cells of Oryza sativa L. cv. ‘Roncarolo’ have been cloned in pBR322. Ten clones with specific digestion patterns have been randomly selected. Nine sequences appear to be organized in a clustered tandem array while one is interpersed in the rice genome. The clones have been used to gather information on: (a) their modulation in cultured cells as compared to whole plant and (b) their distribution in different rice cultivars belonging to the Japonica or Indica subspecies of Oryza sativa L. Hybridization with nuclear DNA isolated either from suspension or from seedlings of the ‘Roncarolo’ cultivar revealed extensive quantitative variations, with most cloned sequences showing amplification (up to 75-fold) in cultured cells. Hybridization with nuclear DNA isolated from seedlings or suspension cultured cells from different cultivars belonging to the Japonica or to the Indica sub-species of O. sativa have shown that (a) amplification also occurs in a similar pattern in the case of DNA from the other tested suspension cultured cell types but not in the case of DNA from seedlings; (b) in some cases the tested sequences show minor but significant variations in different rice accessions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290085
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  • 4
    Electronic Resource
    Electronic Resource
    A repeated chromosomal DNA sequence is amplified as a circular extrachromosomal molecule in rice (Oryza sativa L.) (1990)
    Springer
    Molecular genetics and genomics 222 (1990), S. 58-64 
    Details
    ISSN: 1617-4623
    Keywords: Bal31 ; DNA amplification ; Extrachromosomal DNA ; Pulsed field gel electrophoresis ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The plasmid pE10 is a pBR322-derived plasmid carrying a 4.5 kb rice (Oryza sativa L.) repeated DNA sequence. The cloned sequence has been shown to be amplified in cultured rice cells. The analysis of practically intact chromosomal rice DNA molecules by pulsed field gel electrophoresis has now shown that the amplification is associated with the appearance of extrachromosomal molecules. In fact, pE10 hybridizes exclusively with unfractionated DNA from leaf protoplasts, while it recognizes predominantly an extrachromosomal DNA molecule (ECD) of about 45 kb and its multiples in the case of protoplasts from cultured cells. Insensitivity to the action of the exonuclease Bal31 suggests that the molecule is circular. Analysis of restriction endonuclease products with both standard horizontal and pulsed field gel electrophoresis suggest that the extrachromosomal DNA, and its chromosomal counterpart, is composed of tandemly repeated units of about 7 kb. Thus, the smaller extrachromosomal circle should contain 6–7 repeats, while the sequence cloned in pE10 is a subset of this repeat. The extrachromosomal DNA represents about 1 % of total rice DNA and its level of amplification is not affected by the different phases of growth in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283023
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    Paper (German National Licenses)
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