ISSN:
1432-2242
Keywords:
Divergent selection
;
Broiler chickens
;
Growth hormone gene polymorphisms
;
Restriction enzyme map
;
DNA fingerprinting
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Two strains of meat-type chickens which had been derived from the same genetic base, but were selected for high or low abdominal fat content, respectively, were analyzed for polymorphisms in the growth hormone gene (GH). A total of four DNA polymorphisms were identified, one at a SacI restriction site and three at MspI restriction sites. Restriction mapping indicated that all polymorphisms were in exons and/or introns and not in flanking regions of the gene. The incidence of GH polymorphisms was determined in 20 chickens from each strain and significant differences were observed for two of the four polymorphisms. Analysis by DNA fingerprinting using (CAC)5 as a probe indicated that the inbreeding coefficient was 0.1 in both strains and that random genetic drift was minimal. Thus, the selection for abdominal fat appears to have affected the frequency of alleles of the growth hormone gene. Whether this is the direct consequence of an altered growth hormone gene on fat metabolism or reflects linkage to an allele of a neighbouring gene remains to be determined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00215031
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