ISSN:
1432-069X
Keywords:
Cyclobutane pyrimidine dimers
;
(6-4) Photoproducts
;
DNA repair
;
Ultraviolet radiation
;
Organ culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary To examine the induction and repair of UV-induced DNA damage, indirect immunofluorescence was performed on UVB-irradiated organ-cultured normal human skin using monoclonal antibodies specific for either cyclobutane pyrimidine dimers or (6-4) photoproducts. Nuclear immunofluorescence of cyclobutane pyrimidine dimers and (6-4) photoproducts were observed in a dosedependent manner after UVB irradiation. The intensity of nuclear immunofluorescence of the upper epidermal layers was stronger and clearer than that of the lower epidermal layers. DNA repair time-course studies showed that both types of DNA damage could be repaired within 24 h after UVB irradiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00375800
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