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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Lasers in medical science 13 (1998), S. 73-77 
    ISSN: 1435-604X
    Keywords: Cartilage ; Chondrocyte ; Laser ; Stress relaxation ; Structure alterations ; Thermal and mechanical effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract It is difficult to permanently alter the shape of cartilage in a controlled way. In otolaryngology and plastic surgery several procedures are done to alter the shape of cartilage, for example the correction of a deviated nasal septum and surgery for bat ears. The aim of this paper is to study the main parameters which are necessary for the phenomenon of reshaping of cartilage under non-destructive laser radiation. We have measured temperature and stress in cartilage when it is being reshaped with a holmium laser, It has been shown that laser-induced stress relaxation in cartilage takes place when the tissue temperature exceeds 70°C. We have determined the conditions which allow the shape of cartilage to be altered without producing any pronounced alteration to matrix structure or chondrocytes.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5044
    Keywords: Agrobacterium ; Dendranthema grandiflora ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In an attempt to develop a system for producing transformed plants from explants ofDendranthema grandiflora, the susceptibility of the cultivar Super White to various wild-type strains ofAgrobacterium tumefaciens andA. rhizogenes was investigated. Tumour formation was not a reliable indicator of the ability of a related disarmed strain to mediate transformation. Following inoculation of explants with disarmedAgrobacterium strains, a number of shoots developed on selective media. However, none of these shoots were transformed. By co-cultivating stem internode explants with a mixed inoculum of wild-type and disarmed strains, it was possible to obtain a callus stably transformed withAgrobacterium carrying a disarmed T-DNA. Histological analysis of explants revealed that shoot regeneration initially occurred from the cells of the epidermis and subsequently from the cortex. However, the cells which were susceptible to T-DNA transfer were confined to the vascular tissue.
    Type of Medium: Electronic Resource
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