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  • 1
    Electronic Resource
    Electronic Resource
    The effect of monensin on intracellular transport and secretion of α-amylase isoenzymes in barley aleurone (1986)
    Springer
    Planta 167 (1986), S. 252-259 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone (enzyme secretion) ; α-Amylase ; Gibberellin and enzyme secretion ; Hordeum (enzymes) ; Monensin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of monensin on the secretion of α-amylase and other enzymes from the aleurone layer of barley (Hordeum vulgare L. cv. Himalaya) was studied by electrophoresis followed by fluorography and by pulse-chase and organelle-isolation experiments. Monensin markedly inhibits the secretion, but not the synthesis, of α-amylase, acid phosphatase, and at least four other proteins from the aleurone layer. Monensin treatment causes α-amylase to accumulate within the protoplast, but its effect on the different α-amylase isoenzymes is not equal. The accumulation of isoenzyme 2 is not influenced by monensin while isoenzymes 1, 3 and 4 are not secreted but rather accumulate in the cell when monensin is included in the incubation medium. The α-amylase and acid-phosphatase activities which accumulate within the aleurone cells following treatment with monensin are localized in an organelle having a buoyant density greater than that of endoplasmic reticulum and less than that of mitochondria. In pulse-chase experiments with [35S]methionine, labelled proteins accumulate in this organelle in the presence of monensin and do not appear in the incubation medium. We conclude that monensin inhibits the secretion of proteins from the barley aleurone layer by influencing their intracellular transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391423
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  • 2
    Electronic Resource
    Electronic Resource
    Flow mechanisms in dense silica-metal oxide-water suspensions (1988)
    Springer
    Rheologica acta 27 (1988), S. 76-81 
    Details
    ISSN: 1435-1528
    Keywords: Densesuspension ; metal oxide ; silica ; settling ; plasticflow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Abstract The rheological properties of dense silica in water suspensions (approx. 50% solids by volume) containing additions of metal oxides were examined. Metal oxides used were ferric, zinc and stannic. To prevent settling, testing was performed in a rheometer which was modified to provide for continual stirring of the materials. Relatively small oxide additions had the effect of thickening the mixtures and making them non-Newtonian. Different rate-limiting steps for flow were identified depending on the particular mixture, testing temperature and shear strain rate. Flow could be described using empirical equations which are identical to those often used to describe plastic flow in solid crystalline materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01372453
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of solids concentration in dense suspensions of silica-iron(III) oxide and water (1988)
    Springer
    Rheologica acta 27 (1988), S. 197-201 
    Details
    ISSN: 1435-1528
    Keywords: Densesuspension ; power-lawflow ; entropy ; iron oxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Abstract The rheological properties of dense suspensions, of silica, iron (III) oxide and water, were studied over a range of solids concentrations using a viscometer, which was modified so as to prevent settling of the solid components. Over the conditions studied, the material behaved according to power—law flow relationships. As the concentrations of silica and iron(III) oxide were increased, an entropy term in the flow equation was identified which had a silica dependent and an iron (III) oxide dependent component. This was attributed to a tendency to order into some form of structural regularity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01331905
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  • 4
    Electronic Resource
    Electronic Resource
    Monensin inhibits the secretion of α-amylase but not polysaccharide slime from seedling tissues ofZea mays (1988)
    Springer
    Protoplasma 142 (1988), S. 36-45 
    Details
    ISSN: 1615-6102
    Keywords: α-Amylase ; Corn (Zea mays) seedlings ; Growth ; Monensin ; Polysaccharide slime ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of monensin on polysaccharide slime secretion by root tips of corn (Zea mays) was studied. Various treatment times and ionophore concentrations were tested: none resulted in inhibition of slime secretion. Because monensin changes the pH of the medium, its effect was also monitored in strongly buffered media and at different pH's. Even in such media, monensin did not inhibit slime secretion. We also measured the effect of the drug after a pulse with [3H]fucose or a pulse followed by a chase. The amount of labeled slimed secreted was not altered by the ionophore. However, 10μM monensin affected the development of root tips and drastically reduced their growth. We showed that monensin inhibits the secretion of α-amylase by the scutellum of the same plantlet. The importance of the nature of the secretory compound in relation to monensin inhibition of its secretion is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273224
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    Paper (German National Licenses)
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