ISSN:
1432-072X
Keywords:
Cytochrome P-450
;
Induction
;
Herbicide
;
Detoxification
;
Isozyme
;
Streptomyces griseolus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The elevated soluble cytochrome P-450 content of Streptomyces griseolus cells found after growth in the presence of sulfonylurea herbicides has been shown to be the result of the appearance of one predominant cytochrome P-450 form. This cytochrome P-450 is the major soluble protein found to increase in amount following herbicide treatment, and functions as part of a sulfometuron methyl hydroxylase system. A second minor inducible cytochrome P-450 has been observed only in cells grown in the presence of chlorimuron ethyl, and a third cytochrome P-450 has been found to be present in all cells independent of the presence of sulfonylurea inducers. The three cytochrome P-450 isozymes are distinguishable primarily by their anion exchange properties; however, spectral properties, substrate inducibility, and enzymatic activity provide several further distinguishing features. The recognition of these inducible, xenobiotic metabolizing cytochromes P-450 in S. griseolus provides the only known description of monooxygenase proteins related to herbicide metabolism in bacteria.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425579
Permalink
