ISSN:
1432-203X
Keywords:
Crassostrea gigas
;
Dictyopteris prolifera
;
Marine brown alga
;
Protoplast
;
Single cell
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 107 cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00231940
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