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  • Artificial insemination from the donor • Legal aspects  (1)
  • Direct immunogold histochemistry  (1)
  • 1
    ISSN: 1432-0568
    Keywords: Mouse embryo day 6 and 7 ; Direct immunogold histochemistry ; Laminin subunits of A- and B1-chains ; Mesoderm formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Using ultrastructural immunogold histochemistry on LR-Gold-embedded 6- and 7-day-old mouse embryos we investigated the appearance of the A- and B1-chains of the laminin molecule during mesoderm formation. With the help of antibodies against the A-chain and the E4 fragment of the B1-chain of the laminin molecule we were able to detect the subunits in vivo. Staining for the E4 fragment of the short arm of the laminin molecule from day 6 was negative. In contrast, strong staining for the A-chain of laminin was observed. Our results show, that the A-chain of laminin appears before the B1-chain in the 6-day-old mouse embryo before a basement membrane is seen between the ectodermal and entodermal cell layers. Furthermore, the staining pattern indicates, that the laminin molecule changes its orientation in the basement membrane of the ectoderm during mesoderm formation. On day 7 staining for the A-chain of laminin and for the E4 fragment was seen in a random distribution throughout the entire basement membrane, whereas in areas were the onset of mesoderm formation was taking place, the E4 fragment was restricted to the edge of the disintegrating basement membrane.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Reproduktionsmedizin 16 (2000), S. 249-252 
    ISSN: 1434-808X
    Keywords: Schlüsselwörter ; Artifizielle donogene Insemination • Rechtsfragen ; Keywords ; Artificial insemination from the donor • Legal aspects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Therapeutical inseminations from the donor take place in Germany for more than tens of years. The 73th German Phycians × Meeting has given consent in 1970. Today more than 60,000 to 70,000 children live in Germany born after fecundation by donor sperm. Selection of spermdonors takes place in aggreement to international criteria. The priciples of treatment of donogen insemination are in hand as recommendation. But some legal problems demand lawfull regulation for instance anonymity of the donor, law of family and successions, safe-keeping of documents, fees for the donor.
    Notes: Zusammenfassung Therapeutische donogene Inseminationen werden seit mehr als 3 Jahrzehnten in Deutschland durchgeführt. Der 73. Deutsche Ärztetag hatte 1970 seine Zustimmung gegeben. Heute leben in Deutschland mehr als 60.000–70.000 Kinder, die ihr Leben der Befruchtung mit Spendersperma verdanken. Die Auswahl der Spermaspender erfolgt nach international üblichen Kriterien. Es liegen Behandlungsgrundsätze für die donogene Insemination als Empfehlungen vor. Aber einige rechtliche Probleme wie Anonymität des Spenders, Familien- und Erbrecht, Verwahrfrist der Akten, Spenderhonorar bedürfen noch einer gesetzlichen Regelung.
    Type of Medium: Electronic Resource
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