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  • 1
    ISSN: 1432-2307
    Keywords: Thyroid medullary carcinoma ; Calcitonin ; EC granule ; Serotonin ; Protein A-colloidal gold
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In two medullary carcinomas of the thyroid gland two types of secretory granules were found electron microscopically in the cytoplasm of the tumour cells. The sizes of the granules in one case ranged 103–345 nm in diameter; they were round in shape, and they co-existed in the same tumour cell. They could not, therefore, be distinctively subdivided into two types. In another case, secretory granules in the cytoplasm closely resemble EC granule in morphology. Using the protein A-colloidal gold (PAG) technique the content of secretory granules could be identified as calcitonin irrespective of their sizes or morphology. Immunoreactivity at the ultrastructural level was fairly well preserved even in the osmium-fixed tumour cells. The labelling index, expressed as a mean number of gold particles per unit square area of the secretory granule, was higher in the non-osmium-fixed tumour cells than in the osmium-fixed. Non-osmium-fixed tumour cells embedded either in epoxy or methacryl resin were almost equally labelled with gold particles. The result indicates that the PAG method is practicable to demonstrate the ultrastructural localization of calcitonin even in the osmium-fixed, epoxy resin embedded material.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-879X
    Keywords: ethane selective oxidation ; acrolein ; Cs–Fe/SiO2 ; aldol condensation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A significant yield of aldehydes was obtained by the partial oxidation of ethane over alkali‐modified Fe/SiO2 catalysts at 475°C (〈2% in the case of Cs–Fe/SiO2). Not only acetaldehyde and formaldehyde but also acrolein were produced in the present system. There are no reports regarding the catalysts for the direct acrolein formation from partial oxidation of ethane. Such significant promoting effect of alkali‐modified Fe/SiO2 catalysts in the partial oxidation of hydrocarbons has never been observed. Aldol‐type condensation for formation of acrolein could occur in the partial oxidation of ethane over alkali‐modified Fe/SiO2 catalysts.
    Type of Medium: Electronic Resource
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