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  • 1
    Electronic Resource
    Electronic Resource
    Efficient transformation of the edible basidiomycete Lentinus edodes with a vector using a glyceraldehyde-3-phosphate dehydrogenase promoter to hygromycin B resistance (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 1047-1052 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsLentinus edodes ; Glyceraldehyde-3-phosphate dehydrogenase gene ; Restriction enzyme-mediated integration (REMI) ; Hygromycin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To construct a vector for high-level expression of heterologous genes in Lentinus edodes, the L. edodes GPD promoter, which is expressed constitutively and strongly, was fused to a hygromycin B phosphotransferase gene (hph) derived from Escherichia coli as a selective marker. Using the resulting pLG-hph construct, L. edodes was efficiently transformed to hygromycin resistance by restriction enzyme-mediated integration (REMI). The restriction enzyme concentrations yielding the maximal numbers of transformants by the REMI method were 10 U per transformation in the case of BglII and 25–50 U in the case of HindIII. Southern analysis of the transformants indicated that some 50% of plasmid integrations were REMI-mediated events. These results indicate that pLG is a useful vector for transformation of L. edodes. Deletion analysis of the GPD promoter region suggested that the segment between positions −442 bp and −270 bp relative to the transcription start point may be essential for function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050033
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Nicorandil increases adenosine 5′-monophosphate-primed interstitial adenosine via activation of ecto-5′-nucleotidase in rat hearts (2000)
    Springer
    Heart and vessels 15 (2000), S. 81-85 
    Details
    ISSN: 1615-2573
    Keywords: Key words Nicorandil ; Adenosine ; Ecto-5′-nucleotidase ; Cyclic guanosine monophosphate ; Microdialysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract With the use of microdialysis techniques, we examined the effects of nicorandil, a hybrid of an ATP-sensitive K+ (KATP) channel opener and a nitrate compound, on the production of interstitial adenosine in rat hearts in situ. The level of dialysate adenosine measured under a constant supply of adenosine 5′-monophosphate (AMP) reflected the activity of endogenous ecto-5′-nucleotidase. Nicorandil (0.3–3 mM) increased the level of AMP (100 μM)-primed dialysate adenosine in a concentration-dependent manner, and this effect was completely abolished by the guanylate cyclase inhibitor, methylene blue (100 μM), but not by the KATP channel blocker, glibenclamide (10 μM). Another KATP channel opener, cromakalim (0.1–1 mM), did not increase the production of AMP-primed dialysate adenosine. These results suggest that nicorandil increases the level of interstitial adenosine via cyclic guanosine monophosphate-mediated activation of ecto-5′-nucleotidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003800070036
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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