ISSN:
1432-0789
Keywords:
Vesicular-arbuscular mycorrhiza
;
Glomus intraradices
;
Ergosterol
;
Fungal biomass
;
High performance liquid chromatography
;
Gas chromatography-mass spectrometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Geosciences
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Ergosta-5,7,22-tri-3-enol (ergosterol) was identified by gas chromatography-mass spectrometry in roots of berseem (Trifolium alexandrinum L., cv. Landsorte) and sweet corn (Zea mays L., cv. Honeycomb-F1) infected with the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus intraradices. The fungal-derived compound ergosterol was determined quantitatively in root extracts using reverse-phase high performance liquid chromatography. The concentrations of ergosterol in VAM-infected roots reached 72 μ g-1 dry material in berseem and 52 μ g-1 in sweet corn after 80 days of growth, whereas concentrations in non-infected roots remained below 8 μ g-1 dry weight. Additionally, phytosterols such as β-sitosterol, campesterol, and stigmasterol were detected in both infected and non-infected roots. Ergosterol, as a characteristic fungal substance, is proposed as an indicator of fungal biomass in the early stages of VAM infection.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00340581
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