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  • Erwinia chrysanthemi  (2)
  • Ferritin  (1)
  • Iron regulation  (1)
  • 1
    Digitale Medien
    Digitale Medien
    A brief history of iron metabolism (1991)
    Springer
    BioMetals 4 (1991), S. 1-6 
    Details
    ISSN: 1572-8773
    Schlagwort(e): Cell respiration ; Cytochromes ; Ferritin ; Transferrin receptor ; Siderophores
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary A concise history of selected aspects of iron metabolism is presented. According to present understanding, the element is known to be required for transport and reduction of O2, for reduction Of CO2, N2 and ribonucleotides, and for other essential cellular processes. The contributions of pioneers in the field, preeminent among them the cell physiologist and biochemist Otto Warburg, are recounted.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01135550
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  • 2
    Digitale Medien
    Digitale Medien
    Iron(III) complexes of chrysobactin, the siderophore ofErwinia chrysanthemi (1992)
    Springer
    BioMetals 5 (1992), S. 29-36 
    Details
    ISSN: 1572-8773
    Schlagwort(e): Erwinia chrysanthemi ; chrysobactin ; catechol ; iron complex ; coordination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The phytopathogenic bacteriumErwinia chrysanthemi produces the monocatecholate siderophore chrysobactin under conditions of iron deprivation. Only the catecholate hydroxyl groups participate in metal coordination, and chrysobactin is therefore unable to provide full 1:1 coordination of Fe(III). The stoichiometry in aqueous solution is a variable dependent on pH and metal/ligand ratio, in addition to being concentration dependent. At neutral pH and concentrations of about 0.1mm, ferric chrysobactin exists as a mixture of bis and tris complexes. Chrysobactin and its isomers form optically active tris complexes. The dominant configuration depends on the chirality of the amino acid to which the catecholate moiety is attached.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01079695
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  • 3
    Digitale Medien
    Digitale Medien
    Expression, isolation and properties of Fur (ferric uptake regulation) protein ofEscherichia coli K 12 (1988)
    Springer
    BioMetals 1 (1988), S. 62-68 
    Details
    ISSN: 1572-8773
    Schlagwort(e): Fur repressor ; Iron regulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary The clonedfur (ferric uptake regulation) gene ofEscherichia coli K12 was ligated to an expression vector which was inducible with nalidixic acid. The Fur protein was isolated in a single step by immobilized metal-ion-affinity chromatography over zinc iminodiacetate agarose. The amino acid composition of the isolated protein agreed with that predicted from the gene sequence and indicated post-transcriptional removal of the N-terminal methionine residue. All four cysteines were shown to be present as thiols. Proteolysis with trypsin and chymotrypsin yielded large fragments identifiable on polyacrylamide gel electrophoresis. Various divalent metal ions were found by a nitrocellulose filter binding assay to effect non-specific interaction of the Fur dimer with DNA with a dissociation constant of 7 × 10−12 M. A much smaller value, 2.5 × 10−17 M, was measured by gel mobility retardation assay for binding of Fur to a DNA fragment containing the operator sequences of the aerobactin promoter.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01128019
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  • 4
    Digitale Medien
    Digitale Medien
    The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha (1991)
    Springer
    Plant and soil 130 (1991), S. 263-271 
    Details
    ISSN: 1573-5036
    Schlagwort(e): bacteria ; Erwinia chrysanthemi ; iron transport ; phytopathogenicity ; Saintpaulia ionantha ; virulence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract To incite a systemic disease on its specific host, Saintpaulia ionantha, the soft-rot Erwinia chrysanthemi strain 3937 requires a functional high affinity iron transport system. Under iron starvation, strain 3937 produces chrysobactin, a novel catechol-type siderophore. Recent advances in the biochemistry and genetics of iron assimilation in E. chrysanthemi are reported. Analysis of leaf intercellular fluid from healthy and infected plants suggests: (i) leaf vessels in which the bacteria develop during infection would be low in free iron and (ii) chrysobactin could be produced in planta.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00011882
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