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Biochemical and histochemical investigation of diurnal variation in cAMP concentration and adenylate cyclase activity in white dutch clover (1979)

Tu, J. C.

Springer

Protoplasma 99 (1979), S. 139-146

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ISSN: 1615-6102

Keywords: Adenylate cyclase ; Cyclic AMP ; Diurnal variation ; Histochemistry ; Clover yellow mosaic virus ; White clover

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Diurnal changes in the concentration of cAMP in white clover were investigated biochemically and histochemically. Biochemical assays showed that a) the concentration of cAMP in healthy leaves was consistently higher than in CYMV-diseased leaves under the same light condition and b) the concentration of cAMP in clover leaves exhibited a diurnal cycle. The diurnal changes in cAMP concentration were present in both healthy and CYMV-diseased clover leaves. Histochemical localization tests indicated that the adenylate cyclase activity could be semi quantilated as more dense reaction products were present in the membrane systems of healthy than CYMV-diseased leaves. The histochemical results were consistent with the biochemical data.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01274075

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Calculation of flows using three-dimensional overlapping grids and multigrid methods (1995)

Tu, J. Y. ; Fuchs, L.

Chichester [u.a.] : Wiley-Blackwell

International Journal for Numerical Methods in Engineering 38 (1995), S. 259-282

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ISSN: 0029-5981

Keywords: Complex geometries ; Numerical accuracy and efficiency ; 3D overlapping grids ; Multigrid methods ; Finite-volume ; Engine flows ; Engineering ; Engineering General

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Mathematics , Technology

Notes: A computational methodology combining overlapping grid techniques with multigrid methods has been developed for three-dimensional flow calculations in or around complex geometries. The computational accuracy, efficiency and capability of the present approach are investigated in this paper. The incompressible Navier-Stokes equations are discretized using a finite volume method on a semi-staggered grid. The discrete problem is solved by a multigrid algorithm. Some numerical examples are chosen for evaluating numerical accuracy: (a) a straight pipe for which the exact solution is known; (b) curved pipes where previous experimental and numerical data are available; (c) an axisymmetric sudden expansion. The performance of the multigrid method on overlapping grids is assessed. Several cases of flows in stationary and time-dependent complex geometries are given to demonstrate the capability and the potential of the methods that we employ.

Additional Material: 20 Ill.