ISSN:
1432-1424
Keywords:
single cardiac Na+ channels
;
open-state kinetics
;
drug-induced blockade
;
(-)-DPI
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Elementary Na+ currents were recorded at 9°C in inside-out patches from cultured neonatal rat heart myocytes. In characterizing the sensitivity of cooled, slowly inactivating cardiac Na+ channels to several antiarrhythmic drugs including propafenone, lidocaine and quinidine, the study aimed to define the role of Na+ inactivation for open channel blockade. In concentrations (1–10 μmol/liter) effective to depressNP o significantly, propafenone completely failed to influence the open state of slowly inactivating Na+ channels. With 1 μmol/liter, τopen changed insignificantly to 96±7% of the control. Even a small number of ultralong openings of 6 msec or longer exceeding τopen of the whole ensemble several-fold and attaining τopen (at −45 mV) in cooled, (-)-DPI-modified, noninactivating Na+ channels proved to be drug resistant and could not be flicker-blocked by 10 μmol/liter propafenone. The same drug concentration induced in(-)-DPI-modified Na+ channels a discrete block with association and dissociation rate constants of 16.1 ± 5.3 × 106 mol−1 sec−1 and 675 ± 25 sec−1, respectively. Quinidine, known to have a considerable affinity for activated Na+ channels, in lower concentrations (5 μmol/liter) left τopen unchanged or reduced, in higher concentrations (10 μmol/liter) τopen only slightly to 81% of the predrug value whereasNP o declined to 30%, but repetitive blocking events during the conducting state could never be observed. Basically the same drug resistance of the open state was seen in cardiac Na+ channels whose open-state kinetics had been modulated by the cytoplasmic presence of F− ions. But in this case, propafenone reduced reopening and selectively abolished a long-lasting open state. This drug action is unlikely related to the inhibitory effect onNP o since hyperpolarization and the accompanying block attenuation did not restore the channel kinetics. It is concluded that cardiac Na+ channels cannot be flicker-blocked by antiarrhythmic drugs unless Na+ inactivation is removed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01871427
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