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  • 1
    Digitale Medien
    Digitale Medien
    Gastric adenoma — carcinoma sequence with special reference to p53 and Ki-ras gene alterations (1995)
    Springer
    Virchows Archiv 427 (1995), S. 119-124 
    Details
    ISSN: 1432-2307
    Schlagwort(e): p53 ; Ki-ras ; Gastric carcinoma ; Immunohistochemistry ; Polymerase chain reaction-single-strand conformational polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract With the aim of detecting the timing of p53 and Ki-ras gene alterations in the gastric adenoma-carcinoma sequence, 19 early gastric adenocarcinomas arising from adenomas were studied. Immunohistochemically, 5 adenocarcinomas were positive for p53; 3 focally and 2 diffusely. The p53 point mutations were detected in a focal area with p53 immunoreactivity in 2 of the 5 p53-positive adenocarcinomas. This indicated that p53 point mutations may play a less crucial part in malignant conversion of adenoma to adenocarcinoma in the stomach than in the colon. No Ki-ras gene mutations at codons 12 and 13 were detected in any lesion. These results suggest that the adenoma-carcinoma sequence in the stomach has a different mechanism from that in the colon.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00196515
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Steroidal saponin production in callus cultures of Solanum aculeatissimum Jacq. (2000)
    Springer
    Plant cell reports 19 (2000), S. 1240-1244 
    Details
    ISSN: 1432-203X
    Schlagwort(e): KeywordsSolanum aculeatissimum ; Steroidal saponins ; Aculeatiside A ; Aculeatiside B ; Callus culture ; AbbreviationsBA: Benzyladenine ; 2,4-D: 2,4-Dichlorophenoxyacetic acid ; HPLC: High-performance liquid chromatography ; IBA: Indol-3-butyric acid ; KIN: Kinetin ; MS: Murashige and Skoog (1962) medium ; NAA: Naphthaleneacetic acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Callus was induced from the epicotyl of S. aculeatissimum, and the relation between culture conditions and the production of steroidal saponins in the callus was studied. The results indicated that the callus produced the steroidal saponins aculeatiside A and B. The highest production of steroidal saponins occurred at the middle of the log phase. Optimal conditions for the production of steroidal saponins were culturing on MS basal medium supplemented with the combination of 0.05 ppm or 0.1 ppm NAA and 10 ppm BA, and fructose as a carbon source, in the dark at 25  °C. Under these optimal conditions, the callus produced 0.8% (per dry weight) steroidal saponins, or 0.32% aculeatiside A and 0.48% aculeatiside B.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990000250
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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