ISSN:
1432-2145
Schlagwort(e):
Pollen development
;
Gene expression
;
Pollen-style interactions
;
Isozymes
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary During pollen formation within anthers of Zea mays, post-meiotic expression of the cat-3 gene was observed from soon after microspore release from the tetrads until microspore mitosis; from the binucleate stage to maturity the cat-1 gene was expressed instead. Gene expression during pollen function, i.e., germination and tube growth, was determined by means of a new approach based on the in vivo comparative analysis of pollinated and non-pollinated silks. In the early autotrophic stage of germinating pollen ADH-1, CAT-1, and GOT-1 were expressed, whereas during further tube elongation only GOT-1 was detected. Pollination did not give rise to pollen-style hybrid enzymatic molecules nor to the induction of new enzymes on either partner. Silks, even when non-pollinated, showed the expression of additional alcohol dehydrogenase and catalase enzymatic activities, specifically ADH-2 and CAT-3. These data support the view that expression of the catalase system in the male gametophyte is stage specific, and suggest that the style may provide support to the elongating pollen tube at the functional as well as at the nutritional level.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00205230
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