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  • Electronic Resource  (5)
  • Genetic variation  (3)
  • Dominant markers  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Contrasting patterns of genetic diversity in two tropical pines: Pinus kesiya (Royle ex Gordon) and P. merkusii (Jungh et De Vriese) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 436-441 
    Details
    ISSN: 1432-2242
    Keywords: Pinus ; Allozymes ; Chloroplast DNA ; Genetic variation ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied allozyme and chloroplast (cp) DNA variation in natural populations of Pinus kesiya and P. merkusii from Thailand and Vietnam. The results showed striking differences between the two species in the amount and distribution of allozyme variation. P. kesiya harboured considerable allozyme variation and showed weak interpopulational differentiation. In contrast, P. merkmii had very low intrapopulational variability but a high level of interpopulational differentiation. The average Nei's genetic distance separating the two species was exceptionally high (0.701) taking into account their close taxonomic placement in the same subsection Sylvestres. The constructed phylogenetic trees revealed very early divergence of P. kesiya and P. merkusii. The present analysis of cpDNA variation also confirmed the dissimilar character of these two species and was compatible with other evidence indicating the outstanding position of P. merkusii as compared to other Asian members of the subsection Sylvestres. Analysis of cpDNA variation in sympatric populations of P. kesiya and P. merkusii revealed that they are pure representatives of the species in question. This result indicates that despite an overlapping distribution P. kesiya and P. merkusii do not hybridise in nature. We suggest that the distinctive character of P. merkusii is a result of an early separation from other Eurasian pines. Despite spatial proximity, P. kesiya and P. merkusii are kept apart by strong reproductive barriers. The low genetic variability of P. merkusii may be explained by previous bottlenecks, reduced gene flow among populations, and an inbreeding due to small population size and asynchronous flowering.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223690
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Contrasting patterns of genetic diversity in two tropical pines: Pinus kesiya (Royle ex Gordon) and P. merkusii (Jungh et De Vriese) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 436-441 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Pinus ; Allozymes ; Chloroplast DNA ; Genetic variation ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   We studied allozyme and chloroplast (cp) DNA variation in natural populations of Pinus kesiya and P. merkusii from Thailand and Vietnam. The results showed striking differences between the two species in the amount and distribution of allozyme variation. P. kesiya harboured considerable allozyme variation and showed weak interpopulational differentiation. In contrast, P. merkusii had very low intrapopulational variability but a high level of interpopulational differentiation. The average Nei's genetic distance separating the two species was exceptionally high (0.701) taking into account their close taxonomic placement in the same subsection Sylvestres. The constructed phylogenetic trees revealed very early divergence of P. kesiya and P. merkusii. The present analysis of cpDNA variation also confirmed the dissimilar character of these two species and was compatible with other evidence indicating the outstanding position of P. merkusii as compared to other Asian members of the subsection Sylvestres. Analysis of cpDNA variation in sympatric populations of P. kesiya and P. merkusii revealed that they are pure representatives of the species in question. This result indicates that despite an overlapping distribution P. kesiya and P. merkusii do not hybridise in nature. We suggest that the distinctive character of P. merkusii is a result of an early separation from other Eurasian pines. Despite spatial proximity, P. kesiya and P. merkusii are kept apart by strong reproductive barriers. The low genetic variability of P. merkusii may be explained by previous bottlenecks, reduced gene flow among populations, and an inbreeding due to small population size and asynchronous flowering.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050146
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    TransgenicArabidopsis tester lines with dominant marker genes (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 365-372 
    Details
    ISSN: 1617-4623
    Keywords: Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The map positions of a set of eight T-DNA insertions in theArabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar),β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). Theneo, hpt andbar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing thehpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172528
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Transgenic Arabidopsis tester lines with dominant marker genes (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 365-372 
    Details
    ISSN: 1617-4623
    Keywords: Key words Arabidopsis thaliana ; Dominant markers ; Map-based cloning ; Recombinant selection ; T-DNA localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar), β-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172528
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Adaptation to salinity inHordeum jubatum L. populations studied using reciprocal transplants (1996)
    Springer
    Plant ecology 123 (1996), S. 65-71 
    Details
    ISSN: 1573-5052
    Keywords: Ecotype ; Genetic variation ; Plant population ; Reciprocal transplant ; Salt tolerance ; Wild barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tillers and seedlings ofHordeum jubatum L. from three sites with contrasting salinity regimes in central Saskatchewan, Canada were reciprocally transplanted in order to examine the tolerance of populations of this species to salinity and related habitat factors. Survival, growth and fecundity of the three populations were controlled more by transplant site characteristics than by genetic differences, i.e. differences among populations at a site tended to be smaller than differences among sites. Survival, growth and reproduction of all three populations were best at the non-saline site. The population originating at the non-saline site showed the poorest growth in the two saline habitats, but still had substantial salt tolerance. Fecundity was greatest when the populations were grow at their site of origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044888
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    Paper (German National Licenses)
    Fulltext
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