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Identification of molecular markers linked to Rdr1, a gene conferring resistance to blackspot in roses (2000)

von Malek, B. ; Weber, W. E ; Debener, T.

Springer

Theoretical and applied genetics 101 (2000), S. 977-983

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ISSN: 1432-2242

Keywords: Key words Blackspot ; Diplocarpon ; Molecular marker ; Resistance gene ; Rosa

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Blackspot resistance in the tetraploid rose genotype 91/100–5 had been characterised previously as a single dominant gene in duplex configuration. In the present study a tetraploid progeny (95/3) segregating for the presence of the blackspot resistance gene Rdr1 were screened with 868 RAPD and 114 AFLP primers/primer combinations. Seven AFLP markers were found to be linked to Rdr1 at distances between 1.1 and 7.6 cM. The most closely linked AFLP marker was cloned and converted into a SCAR marker that could be screened in a larger population than the original AFLP and was linked at a distance of 0.76 cM. The cloned fragment was used as an RFLP probe to locate the marker on a chromosome map of diploid roses. This is the first report of markers linked to a resistance gene in roses, and the possibilities of using them for a marker-assisted selection for blackspot resistance as well as for map-based cloning approaches are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220051570

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Klinik und bildgebende Diagnostik der Sarkoidose des Nervensystems (2000)

Woitalla, D. ; Henkes, H. ; Felber, S. ; [et al.]

Springer

Der Radiologe 40 (2000), S. 1064-1076

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ISSN: 1432-2102

Keywords: Schlüsselwörter Sarkoidose ; Neurosarkoidose ; Granulom ; MRT ; Keywords Sarcoidosis ; Sarcoidosis complications ; Neurosarcoidosis ; Granuloma ; Brain diseases complications ; Magnetic resonance imaging

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Description / Table of Contents: Abstract The central nervous system is frequently affected during the course of sarcoidosis. Many of these lesions remain without clinical correlates. Unenhanced and contrast enhanced CT and MRI play an important role during the diagnostic work-up of patients with sarcoidosis and suspected or proved CNS involvement. This article summarizes the most frequent manifestations of neurosarcoidosis and provides illustrative examples of MRI findings.

Notes: Zusammenfassung Das Nervensystem ist bei der Sarkoidose häufiger mitbeteiligt als nach klinischen Gesichtspunkten zu vermuten wäre. Den bildgebenden Untersuchungsverfahren kommt eine große Bedeutung beim Nachweis der pathologischen Veränderungen zu. Insbesondere die Kernspintomographie sichert die Diagnosestellung und dient der Verlaufsbeurteilung. In dieser Übersichtsarbeit werden die typischen klinischen und bildgebenden Befunde bei der Neurosarkoidose vorgestellt.