ISSN:
1432-1912
Keywords:
Haemolysis
;
Guinea-Pig Erythrocytes
;
Rat Erythrocytes
;
Phospholipase A
;
Direct Lytic Factor
;
Cobra Venom
;
HÄmolyse
;
Meerschweinchenerythrocyten
;
Rattenery-throcyten
;
Phospholipase A
;
Direkt Lytischer Faktor
;
Cobragift
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 1. At high concentrations phospholipase A of cobra venom has a direct haemolytic action on washed guinea-pig red cells. The concentration necessary for this effect is one hundred times higher than that required for haemolysis in the presence of the direct lytic factor (DLF). 2. Like guinea-pig red cells, rat erythrocytes are lysed by DLF, but are much less sensitive. A potentiation of haemolysis by the combined action of Phospholipase A and DLF is also seen in rat erythrocytes. Concentrations of DLF 50 times higher and of Phospholipase A 100 times higher than used for guinea-pig red cells are necessary to obtain comparable degrees of haemolysis with rat erythrocytes. 3. The degree of potentiated haemolysis depends on both, the concentration of DLF and the concentration of Phospholipase A. The different susceptibility of red cells from different species to haemolysis by cobra venom thus does not depend on a different sensitivity to one of the two haemolysins alone.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00997170
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