ISSN:
0006-3592
Keywords:
parameter estimation
;
enzyme kinetics
;
identifiability
;
Haldanes
;
rate constants
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
For an enzymatic reaction the rate constants in the assumed mechanism (k1, k-1, etc.) sometimes can be calculated from the steady-state parameter values (Vmax, Km, etc.) and sometimes cannot. When identifiability problems occur, these are obscured by redundancy occurring among the steady-state parameters. This redundancy is only partly revealed by the known Haldane relations. We found the additional constraints between the parameters. These relations allow to predict in which situation rate constants are identifiable by steady-state kinetic methods. © 1996 John Wiley & Sons, Inc.
Additional Material:
2 Tab.
Type of Medium:
Electronic Resource
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