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  • 1980-1984  (3)
  • ATPase cytochemistry  (2)
  • Halophyte  (1)
  • 1
    ISSN: 1432-2048
    Keywords: Halophyte ; Ion accumulation ; Suaeda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 104 (1980), S. 55-65 
    ISSN: 1615-6102
    Keywords: ATPase cytochemistry ; Phloem ; Ricinus communis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Standard lead precipitation procedures have been used to examine the localization of ATPase activity in phloem tissues ofRicinus communis. Reaction product was localized on the plasma membrane of the companion cells associated with sieve elements and of parenchyma cells in phloem tissues from the leaf, petiole, stem and root. ATPase activity was also present on the plasma membrane and dispersed P-protein of sieve elements in petiole, stem and root tissue, but was absent from the plasma membrane of these cells in the leaf minor veins. Substitution ofβ-glycerophosphate for ATP produced no change in the localization of reaction product in leaf tissue. These findings are discussed in relation to current theories on the mechanism of sugar transport and phloem loading.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6102
    Keywords: Analytical electron microscopy ; ATPase cytochemistry ; Plasma membrane ; Ricinus communis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The claim that osmium-containing deposits which lack lead are frequently and incorrectly interpreted as enzymatic reaction products in lead precipitation techniques for ATPase localization in plants is without foundation. Proper controls clearly demonstrate the enzymatic origin of membrane-located deposits and the presence of lead is confirmed by analytical electron microscopy.
    Type of Medium: Electronic Resource
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