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  • 1
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Journal of Biotechnology 38 (1994), S. 81-88 
    ISSN: 0168-1656
    Schlagwort(e): Heterologous protein ; S. cerevisiae ; [abr] %PI(-); percentage of cells do not stain with propidium iodide ; [abr] ADC1; gene encoding the yeast alcohol dehydrogenase I (promoter) ; [abr] AP(CE); AP activity measured in the soluble protein cell extract ; [abr] AP(M); AP activity measured in the culture medium as U ml^-^1 ; [abr] BCK1/SLK1; gene encoding the yeast protein kinase Bck1/Slk1 ; [abr] CAT(CE); CAT activity measured in the soluble protein cell ; [abr] CAT(M); CAT activity measured in the culture medium as U ml^-^1 ; [abr] CYC1; gene encoding the yeast cytochrome oxidase I (terminator) ; [abr] MKK1/MKK2; genes encoding the yeast kinases Mkk1 and Mkk2 ; [abr] PEP4; gene encoding the yeast proteinase A ; [abr] PKC1; gene encoding the yeast kinase C ; [abr] PRB1; gene encoding the yeast proteinase B ; [abr] SLT2/MPK1; gene encoding the yeast MAP kinase homolog Slt2/Mpk1 ; slt2 mutant
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 121 (1979), S. 265-270 
    ISSN: 1432-072X
    Schlagwort(e): 1,6-β-Glucanase ; Characterization ; Catabolite repression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-β-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-β-glucanase, α-amylase and β-glucosidase activities. Only four proteins, one associated with the enzyme, were found by polyacrylamide gel electrophoresis under non denaturing conditions. The enzyme behaves as an acidic protein (pI 4.65) with an optimum pH of 5 and an endohydrolytic mode of action. The activity was lost at pHs greater than 7. The enzyme was also found associated with the mycelium. Its synthesis was repressed by glucose or growth-promoting sugars. Derepression in low glucose containing medium required protein synthesis. 8-Hydroxyquinoline, an RNA synthesis inhibitor, added during the derepression period did permit some increase in the specific activity but prevented it when added at the beginning of that period.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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