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  • High-performance TLC  (2)
  • Key words. Late replication banding; DAPI; actinomycin D; frogs.  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Fluorescence replication banding of frog chromosomes (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 73-77 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Late replication banding; DAPI; actinomycin D; frogs.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. To identify individual chromosomes of a frog karyotype by their fluorescence banding patterns, chromosomes were stained with actinomycin D and 4,6-diamidino-2-phenylindole (DAPI) after incorporation of BrdU during the late S-phase. The chromosomes of three Rana species which were selected for this study (R. ridibunda, R. lessonae and R. japonica) showed well-defined late replication bands. The fluorescence patterns obtained were the reverse of those produced by a 4Na-EDTA Giemsa-staining technique. Fluorescence patterns of the two water frog species (R. ridibunda and R. lessonae) were similar to each other, except for the different fluorescence of the centromeric heterochromatin, which gave extremely bright signals in R. ridibunda but no signal in R. lessonae. Experiments also showed differences between the fluorescence patterns of R. lessonae chromosome 13 in the Italian and Luxembourgian populations. These results sho w that the fluorescence replication banding using actinomycin D and DAPI is very effective in identifying individual frog chromosomes and detecting their structural changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00000582
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Reversed-phase, high-performance, thin-layer chromatography of metal tetrakis (p-tolyl) porphine complexes on octadecylbonded stationary phase (1985)
    Springer
    Chromatographia 20 (1985), S. 49-52 
    Details
    ISSN: 1612-1112
    Keywords: High-performance TLC ; Metal complexes ; Tetrakis (4-methylphenyl) porphine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The metal complexes ofmeso-tetrakis (p-tolyl) porphine (TTP) are chromatographed on octadecyl-bonded silica gel thin-layer with various organic solvents of relatively high polarity used as the eluent. The mobility of the metal-TTP complex depends on its centralmetal, increasing in the order of Cu(II)〈Pd(II)≈NI(II)〈TTP free acid〈V(IV) as vanadyl〈Zn(II)〈Mg(II), although some irregularities are found with the different developing solvents used. The concept of Hildebrand's solubility parameter is applied to the discussion of the mobility of each compound, on the solvents used as the developer. Successful separation of the TTP compounds is performed by using a mixture of acetone and acetonitrile as the developer, although the Ni- and Pd-complexes are not completely resolved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02260487
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    High-performance thin-layer chromatography of metal complexes ofmeso-tetrakis(p-tolyl)porphyrin on cellulose and silica gel (1984)
    Springer
    Chromatographia 18 (1984), S. 441-444 
    Details
    ISSN: 1612-1112
    Keywords: High-performance TLC ; metal complexes ; Tetrakis(4-methylphenyl)porphyrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The migration behavior of metal complexes ofmeso-tetrakis(p-tolyl)porphyrin (TTP) are studied in HPTLC systems with cellulose and silica gel thin-layers and various organic solvents of relatively low polarity. The mobility of the metal complex tends to increase in the following orders of its central-metal elements: on cellulose, Mg(II)〈Zn(II)〈V(IV)〈(H)〈Pd(II)〈 Ni(II)≈Cu(II); on silica gel, Mg(II)〈V(IV)〈Zn(II)= (H)〈Ni(II)≈Pd(II)⩽Cu(II). Recommendable phase system for the separation of metal-TTP complexes is a combination of silica gel thin-layer with a carbon disulfide-m-xylene mixture though the complexes of Ni, Cu and Pd are not completely resolved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262974
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    Paper (German National Licenses)
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