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  • 1995-1999  (2)
  • Hopkinson bar  (1)
  • Keywords: ethanol; recombinant; E. coli KO11; lignocellulosic; chemostat; stability  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Comparative stability of ethanol production by Escherichia coliKO11 in batch and chemostat culture (1999)
    Springer
    Journal of industrial microbiology and biotechnology 23 (1999), S. 701-708 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: ethanol; recombinant; E. coli KO11; lignocellulosic; chemostat; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Differing claims regarding the stability of the recombinant ethanologen E. coli KO11 are addressed here in batch and chemostat culture. In repeat batch culture, the organism was stable on glucose, mannose, xylose and galactose for at least three serial transfers, even in the absence of a selective antibiotic. Chemostat cultures on glucose were remarkably stable, but on mannose, xylose and a xylose/glucose mixture, they progressively lost their hyperethanologenicity. On xylose, the loss was irreversible, indicating genetic instability. The loss of hyperethanologenicity was accompanied by the production of high concentrations of acetic acid and by increasing biomass yields, suggesting that the higher ATP yield associated with acetate production may foster the growth of acetate-producing revertant strains. Plate counts on high chloramphenicol-containing medium, whether directly, or following preliminary growth on non-selective medium, were not a reliable indicator of high ethanologenicity during chemostat culture. In batch culture, the organism appeared to retain its promise for ethanol production from lignocellulosics and concerns that antibiotics may need to be included in all media appear unfounded.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900690
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Constitutive Behavior of Epon 828/T-403 at Various Strain Rates (1998)
    Springer
    Mechanics of time-dependent materials 2 (1998), S. 103-111 
    Details
    ISSN: 1573-2738
    Keywords: dynamic response ; Epon 828 ; T-403 ; epoxy ; high-strain rate test ; Hopkinson bar ; material modeling ; strain-rate effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The uniaxial compressive mechanical response of an epoxy, Epon 828/T-403, was experimentally, measured over a strain-rate range of 1.1 × 10-4 to 5.2 × 103 s-1. A modified split Hopkinson pressure bar was employed to apply dynamic compressive loading over a very short time of ∼ 0.2 millisecond, whereas an MTS was used to conduct quasi-static experiments at a duration of 0.2 to 2,000 seconds to determine strain-rate sensitivity. The experimental results show that the compressive strength of the epoxy increases with increasing strain rate until adiabatic heating offsets the strain-rate hardening. A constitutive model based on the Johnson–Cook model was constructed to describe the stress-strain behavior of the epoxy at the strain rates tested. A Ludwig equation was modified to model the stress-strain behavior at a reference strain rate, which included elastic deformation, a yield-like peak, and a strain-softening region. A hyperbolic tangent function provided a good description of the strain-rate effect. The material constants in this proposed model were determined using the experimental results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009866108965
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    Paper (German National Licenses)
    Fulltext
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