ISSN:
1432-0533
Keywords:
Human pituitary adenomas
;
Tissue culture
;
Cell culture
;
Ultrastructure
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Pituitary adenomas of 120 patients were investigated in tissue and in cell cultures. Under different conditions of culture, the biopsies revealed different rates of growth. In tissue cultures 66% of the samples could be propagated while tumour specimens explanted in cell suspensions proliferated in 80% of the cases. Attempts to establish subcultures were also more successful when specimens were put into cell cultures primarily. Further differences of growth characteristics were observed in connection with the histological types of the parent tissues. According to the old terminology cells of mixed type adenomas had the longest life-span both in tissue and in cell cultures. Considering the proliferative activity, this type of tumour could be most frequently subcultured. Concerning the relationship of different cell components of the biopsies it was proven that survival and proliferation of adenoma cells may occur without fibroblasts. The appearance of fibroblast-like cells in older cultures is a morphological manifestation of the senescence of the specific cell types. Qualitative features of cultured adenoma cells showed that on the basis of cytomorphological properties “chromophobe” samples could be separated from the other types. Cells of acidophil and mixed type growths had the capacity of developing in vitro various migratory shapes, while “chromophobe” cells did not possess this ability. The histological diagnoses made independently from cultures confirmed the tissue culture findings. Differences of in vitro characteristics correlated also with the ultrastructural features of the cultured adenoma cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00707110
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