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  • Hymenoptera  (3)
  • Astrocytomas culture  (1)
  • Biopsy  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Flowcytometric and cytogenetic analysis of human cultured cell lines derived from high- and low-grade astrocytomas (1983)
    Springer
    Acta neuropathologica 60 (1983), S. 40-48 
    Details
    ISSN: 1432-0533
    Keywords: Cytogenetics ; Flowcytometry ; T/E ratio ; Astrocytomas culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five human cell lines cultured from high- and low-grade astrocytomas in cerebral hemisphere have been analyzed for DNA and protein distribution by flowcytometric (FCM) and correlated with cytogenetic profiles. Simultaneous calibration with chicken erythrocytes as a co-running standard provided an estimate of chromosomal number of predominate stem cells of each cell line by the ratio of the DNA content of the major peak (G1) to that of chicken erythrocyte (T/E ratio) of FCM. Various lines had different distributions of chromosomal number, ranging from near diploid to tetraploid. Each line had a stem-cell population and chromosomal markers indicative of clonal selection, but no common marker specific to astrocytomas. The histogram of DNA distribution obtained by FCM correlated well with the chromosomal distribution by cytogenetic analysis. In addition, simultaneous measurement of protein and DNA content in multidimensional FCM demonstrated a sigmoid configuration of the profiles, which indicated a gradual increase of protein content associated with an increase of chromosomal number or with progression of cell cycle. To avoid confusion of a bimodal chromosomal distribution with the G2/M phase of the cell cycle, and to determine chromosomal numbers associated with a DNA histogram, simultaneous cytogenetic and FCM study are required. More rapid than cytogenetic analysis, the T/E ratio allows estimation of chromosomal number of the stem-cell population associated with DNA histograms of cultured glioma-derived cell lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685346
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunofluorescent staining of frozen sections for glial fibrillary acidic protein (1982)
    Springer
    Acta neuropathologica 58 (1982), S. 69-72 
    Details
    ISSN: 1432-0533
    Keywords: GFAP ; Glioma ; Biopsy ; Astrocytoma ; CNS tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunofluorescent staining for glial fibrillary acidic protein (GFAP) has been used as part of the diagnostic evaluation of eleven patients and compared with routine special stains. In one case, a difficult fibrillary neoplasm of the spinal cord, this diagnostic procedure provided rapid, positive identification of the glial nature of the tumor. In all cases, the GFAP reactivity was consistent with staining properties of PTAH and more rapid than PTAH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692700
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Identification and bioassay of macrocyclic lactone sex pheromone of the halictine beeLasioglossum zephyrum (1985)
    Springer
    Journal of chemical ecology 11 (1985), S. 1447-1456 
    Details
    ISSN: 1573-1561
    Keywords: Lasioglossum zephyrum ; Halictidae ; Hymenoptera ; macrocyclic lactones ; sex pheromones ; kin recognition pheromones ; Dufour's gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The macrocyclic lactones found in the Dufour's gland of the halictine beeLasioglossum zephyrum are female sex pheromones. Octadecanolide, eicosanolide, docosanolide, and tetracosanolide, as well as monounsaturated homologs of each, are components of the Dufour's gland secretion of this species. Furthermore, a series of odd-carbon-numbered saturated and unsaturated hydrocarbons and isopentenyl docosanoate occur in extracts of the Dufour's gland and of whole females. Two different mixtures of all four synthetic lactones, and additionally a treatment consisting of the natural extract, elicit higher response levels than two of the lactones separately or either of the controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01012144
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Formic acid in caustic cephalic secretions of stingless bee,Oxytrigona (Hymenoptera: Apidae) (1987)
    Springer
    Journal of chemical ecology 13 (1987), S. 1079-1086 
    Details
    ISSN: 1573-1561
    Keywords: Defensive secretions ; formic acid ; Oxytrigona ; Hymenoptera ; Apidae ; stingless bees ; mandibular glands ; Meliponinae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The cephalic extracts of two species in the stingless bee genusOxytrigona were analyzed. Extracts made in diethyl ether, and then derivatized with diphenyldiazomethane, revealed large quantities of formic acid, potentially a major defensive secretion of this stingless bee group. We additionally identify several hydrocarbons, aldehydes, ketones, and acetates. Novel diketones identified by others were detected in both species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01020539
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Mandibular glands of stingless bees (Hymenoptera: Apidae): Chemical analysis of their contents and biological function in two species ofMelipona (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 1465-1472 
    Details
    ISSN: 1573-1561
    Keywords: Melipona ; Hymenoptera ; Apidae ; mandibular glands ; 2-heptanol ; skatole ; nerol ; undecane ; alarm response ; stingless bees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Workers ofMelipona fasciata andM. interrupta triplaridis respond to their respective mandibular gland extracts with alarm recruitment and defensive behavior. Workers rapidly exit from the nest entrance, land on an intruding object, and bite with the mandibles while vibrating the flight muscles. These behaviors are accompanied by the release of the contents of the mandibular glands. Colonies of both species exhibited greater response to their own mandibular gland extracts than to those of other stingless bee species. Chemical analysis identified 2-heptanol as the major component in hexane extracts of each species. Undecane was a constituent of both species; skatole and nerol were identified only in extracts ofM. i. triplaridis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00988512
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    Paper (German National Licenses)
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