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  • 1
    ISSN: 1432-0568
    Keywords: Prolactin cell ; Met-enkephalin ; Immunocytochemistry ; Ultrastructure ; Morphometr ; Rat(Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A stimulatory effect on prolactin secretion had been describe after acute and systemic administration of met-enkephalin, but the effects of this opioid after chronic administration has not been reported, and the response of mammotroph cells is not clear. As a complement to previous studies, a morphometric analysis (light and electron microscopy) was carried out on prolactin cells from female rats treated chronically with met-enkephalin. Clear features of cellular hyperactivity appeared after chronic and systemic administration of the opioid, and these persisted for two weeks. The changes consisted in increases of cellular, cytoplasmic and nuclear areas, volume and surface densities of the Golgi complex and rough endoplasmic reticulum, as well as the numbers of exocytotic figures. These morphological alterations were paralleled by an increase in serum prolactin levels as detected by RIA. It is concluded that the increase in the synthesis and secretory activity of prolactin cells following chronic and systemic administration of met-enkephalin is very similar to those observed after acute and intraventricular administration.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0568
    Keywords: Prolactin ; Met-enkephalin ; Estradiol ; Immunocytochemistry ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to elucidate the possible role of estrogens in the sex-dependent modulation of met-enkephalin-induced prolactin secretion, and the cellular response at adenohypophyseal level, analyses of serum prolactin levels together with a morphometric study of prolactin-immunoreactive cells were carried out following intraventricular administration of the opioid to estrogenprimed male rats. Administration of the opioid led to an increase in the serum levels of the hormone and, morphometrically, a significant increase in the cellular, cytoplasmic and nuclear areas was observed. When naloxone was administered previously, the above, mentioned changes did not appear, and the values found were similar to those observed in the animals treated only with estrogens. The results suggest that the met-enkephalin-induced prolactin secretion is modulated by the gonadal steroid environment.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 29 (1991), S. 441-446 
    ISSN: 1741-0444
    Keywords: Bone conductivity ; Bone impedance ; Bone permittivity ; Cortical bone ; Femoral diaphysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract A preliminary study is presented of the variability of the electric properties, in the axial, tangential and radial directions, as a function of position in the diaphysis of a femoral bovine bone. The measurements were carried out at three frequencies: 100 Hz, 10 kHz and 1 MHz. It is shown that both the conductivity and the permittivity exhibit significant variations along the diaphysis, and increase in magnitude towards the epiphyses. From this study, the variation of the electric properties cannot be clearly and directly ascribed to the longitudinal variability of the total volumetric fluid content of the bone. The results reflect the orthotropic nature of the electric properties, at any given location, and indicate a position-dependent tendency towards axis symmetry.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 26 (1988), S. 313-316 
    ISSN: 1741-0444
    Keywords: Bone conductivity ; Bone impedance ; Bone permittivity ; Diaphysis ; Epiphysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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