ISSN:
1434-4726
Keywords:
Facial nerve
;
Fallopian canal
;
Herpes simplex infection
;
Immunohistochemistry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary An experimental protocol was developed to study viral infection of the facial nerve. Facial palsy was induced in a guinea pig model by inoculating type 1 herpes simplex virus (HSV-1) directly onto the facial nerve in the temporal bone. Partial removal of the bony wall of the fallopian canal was effected, and virus was placed on the nerve after incising its sheath on the right side and without incision of the sheath on the left side. All animals exhibited subsequent bilateral facial palsies, but with severe changes occurring on the right side. The animals were then sacrificed sequentially following infection. Immunofluorescence and histopathological studies revealed the presence of HSV antigen, infiltration of inflammatory cells, hemorrhage, and degeneration of the right facial nerve. In the left nerves, a slight hemorrhage was recognized without cellular infiltration or HSV antigens. These results suggest that the intact facial nerve is rather resistant to HSV infection, but this defense mechanism is easily destroyed by damage to the facial nerve sheath.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00175970
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