ISSN:
1573-0603
Keywords:
Bacterial adherence
;
Bacterial invasion
;
Bacteriological techniques
;
Invasion assay
;
Microbial colony count
;
Soft agar
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Many bacteriologic studies, including cellular invasion and adherence assays, require enumeration of viable organisms or colony forming units. When attempting to screen large numbers of clinical or environmental isolates or laboratory-derived mutants for differences in invasion or adherence phenotype, standard plating methods can be cumbersome and severely limit the number of organisms or conditions which can be tested. As a potential alternative, we describe a simple, rapid and inexpensive soft agar-based technique for semi-quantitative determination of bacterial colony counts directly within the wells of a 96-well microtiter plate.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009830606819
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