ISSN:
1432-203X
Keywords:
KeywordsSolanum aculeatissimum
;
Steroidal saponins
;
Aculeatiside A
;
Aculeatiside B
;
Callus culture
;
AbbreviationsBA: Benzyladenine
;
2,4-D: 2,4-Dichlorophenoxyacetic acid
;
HPLC: High-performance liquid chromatography
;
IBA: Indol-3-butyric acid
;
KIN: Kinetin
;
MS: Murashige and Skoog (1962) medium
;
NAA: Naphthaleneacetic acid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Callus was induced from the epicotyl of S. aculeatissimum, and the relation between culture conditions and the production of steroidal saponins in the callus was studied. The results indicated that the callus produced the steroidal saponins aculeatiside A and B. The highest production of steroidal saponins occurred at the middle of the log phase. Optimal conditions for the production of steroidal saponins were culturing on MS basal medium supplemented with the combination of 0.05 ppm or 0.1 ppm NAA and 10 ppm BA, and fructose as a carbon source, in the dark at 25 °C. Under these optimal conditions, the callus produced 0.8% (per dry weight) steroidal saponins, or 0.32% aculeatiside A and 0.48% aculeatiside B.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990000250
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