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  • 1
    Electronic Resource
    Electronic Resource
    PD1, an S-like RNase gene from a self-incompatible cultivar of almond (2000)
    Springer
    Plant cell reports 19 (2000), S. 1108-1114 
    Details
    ISSN: 1432-203X
    Keywords: Key words Almond ; cDNA cloning ; Prunus dulcis Sequence comparison ; S-like RNase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Many flowering plants contain stylar S-RNases that are involved in self-incompatibility and S-like RNases of which the biological function is uncertain. This paper reports the deduced amino acid sequence of an S-like RNase gene (PD1) from the self-incompatible plant Prunus dulcis (almond). The amino acid sequence of PD1, which was derived from cDNA and genomic DNA clones, showed 34–86% identity to acidic plant S-like RNases reported so far, with the highest degree of similarity being to an S-like RNase from Japanese pear (Pyrus pyrifolia). Based on RNA hybridisation experiments it appears that, like for many other S-like RNases, the expression of PD1 is not pistil-specific. Analysis of the genomic structure revealed the presence of three introns, of which one is similar in location to that of the related S-RNase gene from Solanaceae and Rosaceae. At least four bands hybridising to PD1 were found upon Southern hybridisation, suggesting the presence of a multigene family of S-like RNase genes in almond. The putative biological function of PD1 is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000235
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic almond (Prunus dulcis Mill.) plants obtained by Agrobacterium-mediated transformation of leaf explants (1999)
    Springer
    Plant cell reports 18 (1999), S. 387-393 
    Details
    ISSN: 1432-203X
    Keywords: Key words Almond ; Prunus ; Transformation ; Agrobacterium ; Adventitious regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Almond (Prunus dulcis Mill.) leaves were transformed with the marker genes gusA (β-glucuronidase) and nptII (neomycin phosphotransferase II) via Agrobacterium-mediated transformation. Bacterial strains and preculture of explants affected efficiency of gene transfer evaluated by transient expression assays. Following transformation, shoots were induced from primary explants on medium without kanamycin and exposed to selection 20 days after cocultivation. From 1419 original leaves, four shoots (A, B, C and D) were obtained that showed amplification of the predicted DNA fragments by polymerase chain reaction (PCR). After micropropagation of these shoots, only those cloned from shoot D gave consistently positive results in histochemical GUS detection and PCR amplification. Southern blot hybridisation confirmed stable transgene integration in clone D, which was also negative in PCR amplification of an Agrobacterium gene. Additional molecular analysis suggested that the remaining three shoots (A, B and C) were chimeric.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050591
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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