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  • 1
    Electronic Resource
    Electronic Resource
    Transgenic insect-resistant maintainer line (IR68899B) for improvement of hybrid rice (1999)
    Springer
    Plant cell reports 18 (1999), S. 572-575 
    Details
    ISSN: 1432-203X
    Keywords: Key words Transgenic maintainer line ; cryIA(b) gene ; Insect resistant ; Hybrid rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterosis has helped to increase rice yield in F1 hybrids by 15–20% beyond the level of inbred semidwarf varieties. For stable yield performance rice hybrids must also possess genetic resistance to biotic stresses. One of these, stem borer, reduces the expected yield of hybrid rice. The truncated synthetic cryIA(b) gene from Bacillus thuringiensis is known to be effective in controlling stem borer. The development of transformation techniques has provided the technology for incorporating this bacterial gene into the rice genome, which has not been possible by conventional breeding methods. We have introduced a new approach of using a transgenic maintainer line for developing an insect-resistant hybrid rice. An elite IRRI maintainer line (IR68899B) has been transformed with the cryIA(b) gene driven by the 35S constitutive promoter using the biolistic method. The integration and expression of the cryIA(b) gene could be demonstrated through Southern and Western blot analyses that have been carried out so far up to the T2 generations. Insect bioassay data showed an enhanced resistance to yellow stem borer in the Bt + transgenic plants. This is the first report of the development of a transgenic maintainer line for use in hybrid rice improvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050624
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1138-1145 
    Details
    ISSN: 1432-2242
    Keywords: Key words Thaumatin-like protein ; PR-5 ; Rhizoctonia solani ; Sheath blight disease ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, ‘Chinsurah Boro II’, ‘IR72’, and ‘IR51500’ were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos. The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051178
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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