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  • Immunocytochemistry  (1)
  • Key words. Defocusing instabilities, homoclinic orbits, coupling instabilities, integrable pdes, birefringent fibers  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nonlinear science 10 (2000), S. 291-331 
    ISSN: 1432-1467
    Keywords: Key words. Defocusing instabilities, homoclinic orbits, coupling instabilities, integrable pdes, birefringent fibers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: nonfocusing instabilities that exist independently of the well-known modulational instability of the focusing NLS equation. The focusing versus defocusing behavior of scalar NLS fields is a well-known model for the corresponding behavior of pulse transmission in optical fibers in the anomalous (focusing) versus normal (defocusing) dispersion regime [19], [20]. For fibers with birefringence (induced by an asymmetry in the cross section), the scalar NLS fields for two orthogonal polarization modes couple nonlinearly [26]. Experiments by Rothenberg [32], [33] have demonstrated a new type of modulational instability in a birefringent normal dispersion fiber, and he proposes this cross-phase coupling instability as a mechanism for the generation of ultrafast, terahertz optical oscillations. In this paper the nonfocusing plane wave instability in an integrable coupled nonlinear Schrödinger (CNLS) partial differential equation system is contrasted with the focusing instability from two perspectives: traditional linearized stability analysis and integrable methods based on periodic inverse spectral theory. The latter approach is a crucial first step toward a nonlinear , nonlocal understanding of this new optical instability analogous to that developed for the focusing modulational instability of the sine-Gordon equations by Ercolani, Forest, and McLaughlin [13], [14], [15], [17] and the scalar NLS equation by Tracy, Chen, and Lee [36], [37], Forest and Lee [18], and McLaughlin, Li, and Overman [23], [24].
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 235 (1984), S. 159-169 
    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Gonadotropic cells ; Immunocytochemistry ; Testosterone binding ; Cryo-ultramicrotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several attempts have been made to localize steroids by means of immunocytological techniques. However, these methods were found inadequate for detecting steroids bound to their receptors. To localize endogenous testosterone (T) in its target cells at the ultrastructural level, an immunocytological technique was performed on ultrathin sections obtained by cryo-ultramicrotomy. T was detected in the pituitary glands obtained from intact male or female rats and castrated rats, but not in castrated + adrenalectomized rats. Animals were also injected either with testosterone, with other steroids (estradiol, progesterone, corticosterone) or with an androgen antagonist (cyproterone acetate). In addition, some ultrathin sections were preincubated either with phosphate buffers of various pH, corticosterone, cyproterone acetate solution, or with T solution. The content of T in the pituitary before and after fixation was measured by radioimmunoassay; it decreased after fixation. T immunoreactivity was localized in the gonadotropic cells only, both in the male and female rats. At the subcellular level, the immunoreactivity was detected in the cytoplasmic matrix and in the nucleus. Immunoreactive T disappeared 1) in rats after castration+adrenalectomy; by means of radioimmunoassay no T was measured in these pituitary glands; 2) in rats injected with 25 (μg/rat of cyproterone acetate; 3) after preincubation of pituitary sections on a drop of cyproterone acetate (1 × 10-6 M). The immunocytological reaction was not modified when the rats were injected with estradiol, progesterone or corticosterone (1 mg/rat), or after preincubation of the sections with corticosterone (1 × 10-3 M), or a buffer solution at pH 7.6. Lower or higher pH values led to a strong decrease in the immunoreactivity. After injection of T (15 μg/rat) the immunocytological reaction was more abundant in the nucleus and less in the cytoplasm. The immunoreactivity was again observed when the sections were preincubated with cyproterone acetate solution and then with T solution. These data suggest that T can be detected by means of immunocytochemistry. It is probably bound to a specific binding site.
    Type of Medium: Electronic Resource
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