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  • Immunocytochemistry  (1)
  • Key words. nonlinear dispersive fibers, nonsoliton pulses, onset of oscillations, hyperbolic shocks  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nonlinear science 8 (1998), S. 43-62 
    ISSN: 1432-1467
    Keywords: Key words. nonlinear dispersive fibers, nonsoliton pulses, onset of oscillations, hyperbolic shocks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Summary. We study the modulation equations for the amplitude and phase of smoothed rectangular pulse initial data for the defocusing nonlinear Schrodinger (NLS) equation in the semiclassical limit, and show that these equations exhibit shock formation. In this way we identify and explain one source for the onset of pulse oscillations in nonlinear fibers whose transmission is modeled by the semiclassical NLS equation. The onset of pulse ripples predicted here develops on the leading and trailing slopes of a smooth pulse, as a consequence of shock formation in the modulation equations. This mechanism for the onset of pulse ripples is distinct, both in the location and timescale, from the scenario pursued by Kodama and Wabnitz [11]: A piecewise linear pulse evolves for distances O(1) down the fiber, beyond which oscillations develop associated with the vanishing of the upper step of the pulse [10]. Here we show that the scenario in [11] is correct, but specific to pure rectangular pulses ; any smoothing of this data fails to obey their scenario, but rather is described by the results presented here. That is, the semiclassical limit of the NLS equation is highly unstable with respect to smooth regularizations of rectangular data. In our analysis, the onset of oscillations is associated with the location of the maximum gradient of the pulse slopes, and onset occurs on the pulse slopes, at short distances down the fiber proportional to the inverse of this maximum gradient. Explicit upper and lower bounds on the initial shock location are derived. We thereby deduce the onset for this source of pulse degradation scales linearly with the pulse width, and scales with the reciprocal square root of the fiber nonlinear coefficient, the pulse power, and the fiber dispersion coefficient.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 235 (1984), S. 159-169 
    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Gonadotropic cells ; Immunocytochemistry ; Testosterone binding ; Cryo-ultramicrotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several attempts have been made to localize steroids by means of immunocytological techniques. However, these methods were found inadequate for detecting steroids bound to their receptors. To localize endogenous testosterone (T) in its target cells at the ultrastructural level, an immunocytological technique was performed on ultrathin sections obtained by cryo-ultramicrotomy. T was detected in the pituitary glands obtained from intact male or female rats and castrated rats, but not in castrated + adrenalectomized rats. Animals were also injected either with testosterone, with other steroids (estradiol, progesterone, corticosterone) or with an androgen antagonist (cyproterone acetate). In addition, some ultrathin sections were preincubated either with phosphate buffers of various pH, corticosterone, cyproterone acetate solution, or with T solution. The content of T in the pituitary before and after fixation was measured by radioimmunoassay; it decreased after fixation. T immunoreactivity was localized in the gonadotropic cells only, both in the male and female rats. At the subcellular level, the immunoreactivity was detected in the cytoplasmic matrix and in the nucleus. Immunoreactive T disappeared 1) in rats after castration+adrenalectomy; by means of radioimmunoassay no T was measured in these pituitary glands; 2) in rats injected with 25 (μg/rat of cyproterone acetate; 3) after preincubation of pituitary sections on a drop of cyproterone acetate (1 × 10-6 M). The immunocytological reaction was not modified when the rats were injected with estradiol, progesterone or corticosterone (1 mg/rat), or after preincubation of the sections with corticosterone (1 × 10-3 M), or a buffer solution at pH 7.6. Lower or higher pH values led to a strong decrease in the immunoreactivity. After injection of T (15 μg/rat) the immunocytological reaction was more abundant in the nucleus and less in the cytoplasm. The immunoreactivity was again observed when the sections were preincubated with cyproterone acetate solution and then with T solution. These data suggest that T can be detected by means of immunocytochemistry. It is probably bound to a specific binding site.
    Type of Medium: Electronic Resource
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