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  • GALLSTONES  (1)
  • Key wordsPetunia hybrida  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell reports 16 (1997), S. 719-724 
    ISSN: 1432-203X
    Schlagwort(e): Key wordsPetunia hybrida ; 5-Azacytidine ; 5-Aza-2′-deoxycytidine ; Hypomethylation ; Shoot-forming cultures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Shoot bud regeneration from Petunia leaf disks was inhibited when they were cultured with the demethylating agents, 5-azacytidine (AzaC) and 5-aza-2′-deoxycytidine (AzadC), in shoot induction (SI) medium. Explants induced shoot primordia if they were transferred after 1 week from the medium containing the drugs to medium without drugs. The fresh weight of leaf disks cultured on SI medium for 2 weeks in the presence of the drugs was 60–80% lower when compared to control shoot-forming cultures. Internode length was reduced when shoots were transferred to phytohormone-free Murashige and Skoog medium containing the drugs. However, no other morphological abnormalities were seen in these shoots, even at 20 µm AzaC or 5 µm AzadC. Coupled restriction enzyme digestion (with HpaII and MspI) and random amplification of genomic DNA was performed to detect the level of methylation of CCGG sites in the DNA of the explants exposed to AzaC and AzadC. Over 15 amplified bands were detectable in the control. Five of these bands were absent in the amplified products when digested DNA from the drug-treated explants was used as the template, showing that hypomethylation of DNA had occurred. This suggests that inhibition of shoot bud formation in the presence of the drugs AzaC and AzadC may be due to the altered methylation status.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Digestive diseases and sciences 43 (1998), S. 940-942 
    ISSN: 1573-2568
    Schlagwort(e): CHOLECYSTITIS ; XANTHOMA ; XANTHOGRANULOMA ; GALLBLADDER ; CARCINOMA ; GALLSTONES
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Xanthogranulomatous cholecystitis exists in asmall but significant proportion of routinecholecystectomy specimens. A few recent reports haveshown a possible association of this disease withcarcinoma of the gallbladder. All cholecystectomizedspecimens were prospectively evaluated over a period oftwo and half years in a single surgical unit to examinethe incidence of xanthogranulomatous cholecystitis and its association, if any, with carcinoma ofthe gallbladder in an area that is prone to gallbladderdiseases. A total of 460 cholecystectomies wereperformed for various gallbladder diseases. Histological confirmation revealed chronic cholecystitis in311 (67.6%) cases, carcinoma of the gallbladder in 62(13.5%), acute cholecystitis in 29 (6.3%),xanthogranulomatous cholecystitis in 41 (8.9%), andxanthogranuloma and carcinoma of the gallbladder in one case(0.2%) only. Almost all cases were suspected to havechronic cholecystitis on clinical and ultrasonographicfeatures. Two specimens on gross examination showed mass lesions, and hence were suspected to becarcinoma of the gallbladder. Subsequent frozen sectionand histopathology demonstrated xanthogranulomatouscholecystitis. Only one case of xanthogranuloma was found to be associated with carcinoma of thegallbladder but no firm association could be establishedbetween xanthogranulomatous cholecystitis and carcinomaof the gallbladder.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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