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  • 1
    Electronic Resource
    Electronic Resource
    Altered development of polysomal RNA activity in chick-pea (Cicer arietinum L.) embryonic axes. Effects of abscisic acid and temperature (1985)
    Springer
    Planta 164 (1985), S. 517-523 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid and RNA translation ; Cicer ; Embryonic axis ; Germination and temperature (seeds) ; Poly (A)RNA ; Protein synthesis ; Temperature and RNA translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The in vitro activity of polysomal polyadenylated RNA (poly(A)RNA) was studied using chick-pea (Cicer arietinum L.) embryonic axes subjected to treatments retarding germination (H2O 30°C and abscisic acid [ABA] 30°C) or inducing a false germination (thiourea 30°C) in which normal protein synthesis and growth did not occur. All treatments induced a smaller proportion of poly(A)RNA compared with the control (H2O 25°C). However, poly(A)RNA obtained in the presence of ABA had a similar in vitro activity to that of the control. The translation of mRNA from embryonic axes germinated at high temperatures was extensively blocked (70%) by methyl-7-guanosine-5′-triphosphate, whereas mRNA translation from axes treated with H2O-25°C and ABA was completely blocked (100%), indicating a greater cap dependence in the latter cases. Polyacrylamide gel electrophoresis showed that ABA and H2O-30°C each induced the synthesis of a polypeptide with an approximate Mr of 32 kDa, probably a germination regulator. It is suggested that ABA and high temperatures could regulate germination at the translational level as well as affecting ionic-exchange properties, as has been previously demonstrated (Hernández-Nistal et al. 1983, Physiol. Plant. 57, 273–278).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395969
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  • 2
    Electronic Resource
    Electronic Resource
    In vivo diagnosis of Kufs’ disease by extracerebral biopsies (1998)
    Springer
    Acta neuropathologica 96 (1998), S. 102-108 
    Details
    ISSN: 1432-0533
    Keywords: Key words Adult ceroid lipofuscinosis ; Kufs’ disease ; Electron microscopy ; Extracerebral biopsies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In almost all of the earlier reported cases of Kufs’ disease, the adult form of ceroid lipofuscinosis, the diagnosis was ascertained by cerebral tissue examination, while peripheral biopsy examination revealed an apparent poor diffusion of specific lipofuscinic deposits, the finger print profiles (FPs). We report the ultrastructural data from skin, muscle and rectal biopsy specimens from two siblings, both still living, who present clinical features of Kufs’ disease. We observed the presence of FPs in locations that differ from the previous classic reports. Our results emphasize the value of extracerebral biopsies for the diagnosis of Kufs’ disease in vivo, and suggest some physiopathological assumptions based on vascular wall involvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050866
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    Paper (German National Licenses)
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