ISSN:
1573-4986
Schlagwort(e):
L2/HNK-1 carbohydrate
;
antibody recognition
;
laminin binding
;
neural migration and process outgrowth
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Abstract The L2/HNK-1 carbohydrate epitope has been shown to carry an unusual 3′-sulfoglucuronic acid linkedO-glycosidically through a neolactosyl-type back bone to a ceramide residue. Using monoclonal antibodies, the same or a closely related epitope has also been detectedN-glycosidically linked to glycoproteins, amongst them several neural cell adhesion molecules. We used synthetic glycolipids carrying sulfated or non-sulfated glucuronic acid attached to ceramide through glycans of different length to show that not only the sulfated glucuronic acid but also the neolactosyl-type backbone is essential for the recognition of the L2/HNK-1 carbohydrate by a monoclonal antibody, its binding to laminin and its role in neural cell migration and outgrowth of processes from neurons and astrocytes.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00731208
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