ISSN:
1432-072X
Keywords:
Laccase
;
Syringic acid
;
Vanillic acid
;
2-methoxy-1,4-benzoquinone
;
Rhizoctonia praticola
;
Trametes versicolor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Laccases (benzenediol: oxygen oxidoreductases, EC 1.10.3.2) from Rhizoctonia praticola and Trametes versicolor formed different products from syringic and vanillic acids at different pH values, but both enzymes generated the same chemicals at a particular pH. The products were separated by thin-layer and high-performance liquid chromatography. Four compounds were determined from syringic acid (m/z 168, 334, 350 and 486) at pH 6.9, but only two of these (m/z 168 and 334) were found at pH 3.5. In the case of vanillic acid two isomeric dimers (m/z 304) and 2-methoxy-1,4-benzoquinone (m/z 138) were identified. The dimers were formed at both pH values (pH 3.5 and 6.9), but 2-methoxy-1,4-benzoquinone appeared only at pH 3.5.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00414446
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