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  • 1
    ISSN: 1573-904X
    Keywords: adinazolam mesylate ; gradient reversed-phase liquid chromatography ; mass spectrometry ; decomposition products assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A gradient high performance liquid chromatographic method was developed to determine degradation products of adinazolam mesylate in a sustained release tablet formulation. Sample preparations were chromatographed on a YMC-Basic column using a formate buffer/acetonitrile gradient with absorbance detection at 254 nm. Adinazolam mesylate was found to degrade at high relative humidity and temperature to form a major product, the 6-aminoquinoline analog, plus numerous other compounds. Five of these compounds were identified and their structures indicate that the solid-state degradation of adinazolam, in the presence of sufficient moisture, involves not only a hydrolytic mechanism, but also an oxidative mechanism. Potential process impurities were resolved from the drug and degradation products. Recovery was near 100% over the 0.5 to 10% range for the major degradate (6-aminoquinoline) and over the 0.5 to 1% range for the other analytes. The method was applied to tablet samples stressed at high relative humidity and temperature. The relative standard deviation of the assay for the 6-aminoquinoline was less than 2% and less than 13% for the minor components. Calculated mass balances (sum of adinazolam plus degradation products in the degraded tablet divided by the same sum in the undegraded tablet) were less than 100% and were dependent on the extent of degradation in the tablet. The average mass balance result obtained for samples that were an average of 9.5% degraded was 95.0 ± 1.5%. It is possible that the decrease in mass balance with increase in percent degradation may be explained by the formation of many components at trace levels due to degradation by various permutations of hydrolytic and oxidative reaction pathways.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-904X
    Keywords: fluphenazine ; stable isotope ; deuterium labeled ; mass spectrometry ; schizophrenics ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 39 (1985), S. 177-182 
    ISSN: 1570-7458
    Keywords: biological control ; brassica ; labiate herbs ; larval feeding ; oviposition ; Lepidoptera ; Pieris brassicae ; Plutella xylostella
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Des plantes médicinales ont traditionnellement été utilisées en association avec des plantes cultivées dans l'espoir que leur odeur repousserait les insectes nuisibles. Des extraits à l'alcool et des essences volatiles de labiées médicinales ont été essayés au laboratoire pour examiner leur effets répulsif et dissuadant pour la ponte de Plutella xylostella et l'alimentation des chenilles de P. xylostella et Pieris brassicae. Les extraits alcooliques d'hysope, de romarin, de sauge, de thym et de trèfle blanc réduisent la ponte de P. xylostella sur des morceaux de feuilles de chou traitées. Les essences volatiles de sauge et de thym réduisent la ponte de P. xylostella sur des morceaux de feuilles de chou dans des expériences de choix. L'alimentation des chenilles de dernier stade de P. xylostella et P. brassicae dans des expériences de choix est réduite par l'application d'extraits alcooliques des labiées médicinales.
    Notes: Abstract Herbs have been traditionally used as intercrops with crop plants on the assumption that their odour repels pest species. Alcohol extracts and essential oils of labiate herbs were tested in the laboratory for deterrent/repellent responses to ovipositing Plutella xylostella (L.) and feeding larvae of P. xylostella and Pieris brassicae L. Alcohol extracts of hyssop, rosemary, sage, thyme and white clover reduce oviposition by P. xylostella on pieces of brassica leaf in dual-choice tests. Essential oils of sage and thyme reduce oviposition on pieces of brassica leaf. Feeding, in dual-choice tests, by final instar P. xylostella and P. brassicae larvae is reduced by application of alcohol extracts of herbs.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    The protein journal 9 (1990), S. 623-632 
    ISSN: 1573-4943
    Keywords: Pancreatic thread protein ; primary structure ; mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Pancreatic thread protein (PTP) forms double helical threads in the neutralpH range after purification, undergoing freely reversible,pH-dependent globule-fibril transformation. The purified bovine PTP consists on SDS gels of two carbohydrate-free polypeptide chains (Grosset al., 1985). Plasma desorption mass spectrometry and amino acid sequence analysis now confirm that bovine PTP contains two disulfide-bonded polypeptides, an A chain of 101 amino acid residues with a molecular weight of 11,073 and a B chain of 35 residues with a molecular weight of 3970. The intact protein exhibits a molecular weight of 15,036, agreeing 〉99.9% with the molecular weight calculated from the sequence. The B chain sequence was determined by gas-phase Edman degradation of the intact polypeptide. The A chain sequence was determined from overlapping peptides generated by cleavage at lysyl, tryptophanyl, and aspartyl-prolyl residues. Based upon the bovine PTP cDNA structure, the two chains of the protein result from cleavage of a single polypeptide with removal of a dipeptide between the NH2-terminal A chain and COOH-terminal B chain. Comparison of bovine PTP with other proteins reveals significant structural relatedness with the single-chain homologues from human and rat pancreas and with the motif associated with Ca2+-dependent carbohydrate recognition domains. The physiological role of PTP has not yet been resolved. The protein is present in very high concentration in pancreatic secretion and it has been detected in brain lesions in Alzheimer's disease and Down syndrome and in regenerating rat pancreatic islets. The present results provide a firm protein base for ongoing molecular, physical-chemical, and structure-function studies of this unusual protein.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 10 (1984), S. 1579-1596 
    ISSN: 1573-1561
    Keywords: Alsophila pometaria(Harris) ; fall cankerworm ; Lepidoptera ; Geometridae ; sex pheromone ; (Z,Z,Z,E)-3,6,9,11-nonadecatetraene ; (Z,Z,Z,Z)-3,6,9,11-nonadecatetraene ; (Z,Z,Z)-3,6,9-nonadecatriene ; synthesis ; hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z,Z,Z,E)-3,6,9,11-Nonadecatetraene and (Z,Z,Z,Z)-3,6,9,11-nonadecatetraene, sex pheromone components ofAlsophila pometaria, were synthesized by stereoselective Wittig reactions and found to be spectroscopically and chromatographically identical to isolated natural material. Flight-tunnel bioassays and field-trapping experiments confirmed that the two tetraenes together with (Z,Z,Z)-3,6,9-nonadecatriene are sex pheromone components. While traps baited with either tetraene individually captured conspecific males in field-trapping experiments, addition of the triene, which captured no males by itself, to either tetraene resulted in synergistic responses.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-1561
    Keywords: Alkaloids ; mass spectrometry ; infrared spectroscopy ; amphibians ; ants ; decahydroquinolines ; quinolizidines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three alkaloids—two minor decahydroquinolines (DHQs) and a major quinolizidine—were detected in an extract of a Brazilian myrmicine ant (Solenopsis (Diplorhoptrum) sp. picea group). One DHQ (3) was identical to a known frog-skin alkaloid, cis-195A (cis-5-methyl-2-propyldecahydroquinoline), while the second DHQ, an isomer of 3, designated 195J, was assigned a tentative cis-2-methyl-5-propyldecahydroquinoline structure (2) based on mass and infrared spectra. The third alkaloid proved identical to the frog-skin alkaloid 195C, for which a structure had not been previously proposed. Mass and infrared spectral analysis, including chemical ionization tandem mass spectrometry, indicated a 4-methyl-6-propylquinolizidine structure (1) for 195C. The four possible diastereomers were synthesized and the (6Z,10E)-4-methyl-6-propylquinolizidine diastereomer (1b) was identical to the natural alkaloid. Skin extracts of a population of a Madagascan mantelline frog contained, among other alkaloids, minor amounts of the same alkaloid triad 1–3 with 1 again predominating. The common occurrence of alkaloids 1–3 in both ant and frog supports the hypothesis that ants are a likely dietary source for sequestered frog-skin alkaloids and brings to six, the alkaloid classes common to ant and frog.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-1561
    Keywords: Alsophila pometaria (Harris) ; fall cankerworm ; Lepidoptera ; Geometridae ; sex pheromone ; (Z,Z,Z)-3,6,9-nonadecatriene ; (Z,Z,Z,E)-3,6,9,11-nonadecatetraene ; (Z,Z,Z,Z)-3,6,9,11-nonadecatetraene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A sex pheromone extract from fall cankerworm moths,Alsophila pometaria, attracted conspecific males in field tests. Four EAG-active components were isolated from the extract and identified by GC-MS, highfield PMR spectroscopy, and microchemical techniques asn-nonadecane (I), (Z,Z,Z)-3,6,9-nonadecatriene (II), (Z,Z,Z,E)-3,6,9,11-nonadecatetraene (III), and (Z,Z,Z,Z)-3,6,9,11-nonadecatetrane (IV). Studies of the behavioral responses of male moths in a flight tunnel to the isolated components showed II, III, and IV were the major components of the sex pheromone. No sex pheromone behavioral responses were observed for I.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 11 (1985), S. 727-756 
    ISSN: 1573-1561
    Keywords: Sex attractants ; Lepidoptera ; Geometridae ; Noctuidae ; stereospecific synthesis ; (3Z,6Z,9Z)-triene hydrocarbons ; (3Z,6Z,9S,10R)-9,10-epoxydienes ; (3Z,6Z,9R, 10S)-9,10-epoxydienes ; electroantennogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male moths belonging to 17 species of Geometridae and nine species of Noctuidae were captured in traps baited with synthetic chemicals as part of a field screening program. The compounds tested were the C18-C22 homologs of: (1) (3Z,6Z,9Z)-triene hydrocarbons; (2) mixtures containing equal quantities of (3Z,6Z)-cis-9,10-expoxydienes, (3Z,92Z)-cis-6,7-epoxydienes, and (6Z,9Z)-cis-3,4-epoxydienes; (3) (3Z,6Z)-9S,10R-epoxydienes; (4) (3Z,6Z)-9R,10S-epoxydienes; and (5) (3Z,6Z,9Z,11E)-nonadecatetraene. Field captures and electroantennographic assays revealed a high degree of specificity in the responses of many species to the synthetic chemicals. In several species the ability of males to discriminate between the 9S,10R and 9R,10S enantiomers of the monoepoxydiene isomers was clearly shown. Synergists and inhibitors were discovered for several of the reported attractants, some of which were not previously known to have semiochemical activity. The geometrid moths captured includedEpirrhoe sperryi (Herbulot),Mesoleuca ruficillata (Guenée),Triphosa haesitata (Guenée),Metanema inatomaria (Guenée),Prochoerodes transversata (Drury),Cabera erythemaria (Guenée),Synaxis jubararia (Hulst),Dysstroma brunneata ethela (Hulst),Eulithes testata (Linnaeus),Sicya macularia (Harris),Xanthorhoe iduata (Guenée),X. abrasaria aquilonaria (Herrich-Schäffer),X. munitata (Hübner),Itame loricaria (Eversmann),Eupithecia annulata (Hulst),E. rovocastaliata (Packard) andE. satyrata dodata (Taylor). The noctuid moths captured includedBleptina caradrinalis (Guenée),Idia américalis (Guenée),I. aemula (Hübner),Rivula propinqualis (Guenée),Lomanaltes eductalis (Walker),Spargaloma sexpunctata (Grote),Caenurgina distincta (Neumuller),Euclidia cuspidea (Hübner), andZale duplicata (Bethune). Six of the nine noctuid species captured belong to three subfamilies for which sex attractants had not been reported previously. Details for the stereospecific synthesis of (3Z,6Z)-cis-9,10-epoxydienes are also reported.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 379 (1998), S. 33-40 
    ISSN: 1573-5117
    Keywords: Lepidoptera ; Crambidae ; aquatic ecology ; aquatic plants ; distribution ; herbivory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An ecological study was conducted in May and June of 1995 and 1996 in South Carolina to determine the factors associated with distributions of aquatic Lepidoptera (Crambidae: Nymphulinae). Larvae were found at 65 lotic and lentic sites in three ecoregions (Piedmont, Sandhills, Coastal Plain). Nine species of aquatic Lepidoptera were collected from 12 species of aquatic vascular macrophytes. One to six plant species were used as hosts, depending on the species of lepidopteran; however, the number of host plants used by a lepidopteran was significantly correlated with the lepidopteran's frequency of occurrence. Significant habitat associations were found for five species. Langessa nomophilalis (Dyar) was found under the widest range of temperature and width and occurred in both lotic and lentic habitats. Munroessa icciusalis (Walker) was found in lotic and lentic habitats and had the widest range of recorded depths. Parapoynx maculalis (Clemens) occurred at stream sites with lentic-like conditions. Parapoynx obscuralis (Grote) occupied the widest range of pH and was restricted to lotic habitats, and P. seminealis (Walker) was found in both lotic and lentic habitats. Additional species, collected at fewer than 8% of sites, included M. gyralis, P. allionealis, Synclita obliteralis, and S. tinealis. Overall, the distributions of aquatic Lepidoptera in South Carolina were nonrandom and predictable on the basis of habitat characteristics.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1617-4623
    Keywords: Bacillus thuringiensis ; Crystal protein ; Activation ; Lepidoptera ; Toxic fragment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The amino acid sequences necessary for entomocidal activity of the CryIA(b) protoxin of Bacillus thuringiensis were determined. Introduction of stop codons behind codons Arg601, Phe604 or Ala607 showed that amino acid residues C-terminal to Ala607 are not required for insecticidal activity and that activation by midgut proteases takes place distal to Ala607. The two shortest polypeptides, deleted for part of the highly conserved β-strand, were prone to proteolytic degradation, explaining their lack of toxicity. Apparently, this β-strand is essential for folding of the molecule into a stable conformation. Proteolytic activation at the N-terminus was investigated by removing the first 28 codons, resulting in a translation product extending from amino acid 29 to 607. This protein appeared to be toxic not only to susceptible insect larvae such as Manduca sexta and Heliothis virescens, but also to Escherichia coli cells. An additional mutant, encoding only amino acid residues 29–429, encompassing the complete putative pore forming domain, but lacking a large part of the receptor-binding domain, was similarly toxic to E. coli cells. This suggests a role for the N-terminal 28 amino acids in rendering the toxin inactive in Bacillus thuringiensis, and indicates that the cytolytic potential of the pore forming domain is only realized after proteolytic removal of these residues by proteases in the insect gut. In line with this hypothesis are results obtained with a mutant protein in which Arg28 at the cleavage site was replaced by Asp. This substitution prevented the protein from being cleaved by trypsin in vitro, and reduced its toxicity to M. sexta larvae.
    Type of Medium: Electronic Resource
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