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  • 1
    ISSN: 1432-0789
    Keywords: Dactyladenia barteri ; Leucaena leucocephala ; Lignin ; Nitrogen mineralization ; Nitrogen leaching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The evolution of mineral and hydrosoluble organic N released from two soils differing in pH and treated with leaves of Leucaena leucocephala (0, 8.3, 16.7, and 33. g kg-1 soil), Dactyladenia barteri (syn. Acioa barteri; 0 and 16.7 g), and their mixtures was studied in the laboratory using the aerobic incubation-leaching method. N mineralization in untreated soils and in soils supplemented with 8.3 g leucaena leaves was 41–53% higher in the soil from Onne (pH 4.7) than in the soil from Ibadan (pH 6.2), but the organic N content was similar with these treatments in the leachates of the soils from both locations. The application of 16.7 or 33.3 g of either or both type of leaves reduced the rate of mineral N production during the first 4 weeks, particularly in soils treated with dactyladenia leaves (C:N=36). After this lag period, N mineralization proceeded at a faster rate in the soil from Ibadan treated with 16.7 or 33.3 g of leucaena leaves (C:N=12), even in the presence of dactyladenia leaves. In Ibadan soil, after 12 weeks, mineral N apprently derived from leaves of both dactyladenia and leucaena averaged 6.3% of the N applied, and organic N from leaves averaged 9.5%. The addition of dactyladenia and leucaena leaves did not increases the mineral N content in the acid soil from Onne but leaching of soluble organic N with addition of 16.7 or 33.3 g of leaves contributed an N-mineralizable pool of 5.9% of the N applied.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 26 (1998), S. 187-193 
    ISSN: 1432-0789
    Keywords: Key words Pruning ; Nitrogen release ; Root zone ; Albizia lebbeck ; Leucaena leucocephala
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of pruning on the dynamics of N release in the root zone of Albizia lebbeck and Leucaena leucocephala was studied using potted soil and minilysimeters with presterilized sand-medium supplied with N-free nutrient solution. Plants were pruned twice at 11 and 13 months and leachates were collected weekly for 16 weeks starting from first pruning, and analysed for mineral N content. Removal of plant shoots reduced nodule and root biomass by some 30–38% and halved nodule N yields, while total N yields did not differ between pruned and unpruned plants. The dynamics of N nutrient in the rhizosphere was also affected by pruning, irrespective of the growth medium. In soil culture, unpruned plants of both species maintained greater levels of total N in their rhizosphere compared to those that were pruned. In sand culture, nitrate-N was by 66–84% the predominant N form in soil leachate across the two sequential prunings. Over the 16 weeks following these prunings of A. lebbeck and L. leucocephala, cumulative mineral N in both forms was significantly higher in the root zone of unpruned plants, and was consistently greater under rhizobial inoculation. Less mineral N was released into the root zone of each species during the period after the second pruning than after the first one. L. leucocephala released significantly more N than A. lebbeck over the sampling period, but net N release beneath both species was lower than 1%, indicating that tree legumes do not release sizeable proportions of their N into root zone, and that pruning reduces their N release still further.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 112 (1988), S. 129-135 
    ISSN: 1573-5036
    Keywords: alfisol ; inoculation ; Leucaena leucocephala ; nitrogen fertilizer ; nitrogen fixation ; nodulation ; Rhizobium ; shoot growth ; total nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Leonard jar, pot and field experiments examined the effects of inoculation and the influence of nitrogen fertilizer on nodulation, nitrogen fixation and growth ofLeucaena leucocephala (Lam.) de Wit at IITA, Ibadan, Nigeria. Leucaena responded to both inoculation and/or nitrogen application. Shoot growth and total N and P of inoculated plants were comparable to those of the highest N treatment, and the values were about 55% greater than those of uninoculated ones. Field data indicated that toal N yields of inoculated leucaena were increased by 50% with 40 or 80 kg ha−1 of N fertilizer. However, N fertilizer depressed N fixation by 56% as was expected from nodule mass data. N-fixation was delayed for about 8 weeks in the plots without N. Application of small amounts of N starter (20 ppm) proved to be beneficial to satisfy the plant need during the early stage of leucaena growth. The rhizobial strains IRc 1045 and IRc 1050 were effective, competitive and survived well in the field one year after their establishment.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5036
    Keywords: difference method ; isotope dilution ; Leucaena leucocephala ; nitrogen fixation ; Rhizobium inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The amount of nitrogen fixed byLeucaena leucocephala (Lam.) de Wit was assessed on an Alfisol at the International Institute of Tropical Agriculture located in southwestern Nigeria. Estimated by the difference method, nitrogen fixation of leucaena inoculated with Rhizobium strain IRc 1045 was 133 kg ha−1 in six months. Inoculation with Rhizobium strain IRc 1050 gave a lower nitrogen fixation of 76 kg ha−1. Fertilization with 40 and 80 kg N ha−1 inhibited nitrogen fixation by 43–76% and 49–71%, respectively. Estimates with the15N dilution method gave nitrogen fixation of 134 kg ha−1 in six months when leucaena was inoculated with Rhizobium strain IRc 1045 and 98 kg ha−1 for leucaena inoculated with Rhizobium strain IRc 1050. This nitrogen fixation represented 34–39% of the plant nitrogen. Inoculated leucaena derived 5–6% of its nitrogen from applied fertilizer and 56–54% from soil.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 112 (1988), S. 137-141 
    ISSN: 1573-5036
    Keywords: alfisol ; egbeda series ; Leucaena leucocephala ; inoculation ; maize ; nitrogen contribution ; nodules ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The nitrogen contribution from the shoot and root system of symbiotically grown leucaena was evaluated in a field experiment on an Alfisol at IITA in Southern Nigeria. Maize in plots that received prunings from inoculated leucaena contained more N and grain yield was increased by 1.9 t.ha.−1. Large quantities of nitrogen were harvested with leucaena prunings (300 kg N ha−1 in six months) but the efficiency of utilization of this nitrogen by maize was low compared to inorganic N fertilizer (ammonium sulphate) at 80 kg N ha−1. Maize yield data indicated that nitrogen in leucaena prunigs was 34 and 45% as efficient as 80 kg N ha−1 of (NH4)2SO4 for uninoculated and inoculated plants with Rhizobium IRc 1045, respectively. In plots where the prunings were removed, the leaf litter and decaying roots and nodules contributed N equivalent of 32 kg ha−1. Twenty-five kg ha−1 was the inorganic N equivalent from nitrogen fixed symbiotically by leucaena when inoculated with Rhizobium strain IRc 1045. Application of prunings from inoculated leucaena resulted in higher soil ogranic C, total N, pH and available NO3.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 112 (1988), S. 121-127 
    ISSN: 1573-5036
    Keywords: alfisol ; allantoin ; inoculation ; Leucaena leucocephala ; mineral nutrition ; nodulation ; nitrogenase activity ; shoot growth ; total nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pot and field experiments carried out at the International Institute of Tropical Agriculture (IITA) and at Fashola, Southwestern Nigeria, examined the effect of inoculation and N, P and micronutrients on nodulation and growth ofLeucaena leucocephala (Lam.) de Wit. In pot studies all parameters measured, except the percentage of nitrogen in shoots, were improved by inoculation, nitrogen and phosphorus. Micronutrients increased only nitrogen and allantoin contents. Interactions between inoculation and P, N and micronutrients on nodulation and growth of leucaena were observed. The effect of inoculation and fertilization with phosphorus or micronutrients was further investigated in field experiments. Establishment of uninoculated and unfertilized leucaena was poor at both locations due to low soil fertility and the presence of only a few native leucaena rhizobia. At one site, only inoculated plants were nodulated, while at the other, all plants produced nodules. Shoot dry weight, total nitrogen and phosphorus of inoculated plants were statistically equal to nitrogen-fertilized plants. Uninoculated plants were stunted. Generally, micronutrients did not influence nodulation, total nitrogen or growth of leucaena. They had only a positive effect on nitrogenase activity. Phosphorus increased total nitrogen and phosphorus uptake and plant growth. A 75% increase in shoot dry weight was obtained when 80 kg P ha−1 was applied to inoculated leucaena with Rhizobium strain IRc 1045. Inoculated plants contained more allantoins than uninoculated ones but no significant correlation was found between these compounds and other parameters of N fixation.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5036
    Keywords: inoculation ; Leucaena leucocephala ; nitrogen fixation ; nodulation ; persistence ; Rhizobium strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Establishment of Leucaena leucocephala was poor at Ibadan (Transition forest-savanna zone) and Fashola (savanna zone, 70 km north of Ibadan) in southwestern Nigeria as a result of low soil fertility and the presence of only a few native rhizobia capable of nodulating it. Inoculation with L. leucocephala at these two locations in 1982 resulted in striking responses with Rhizobium strains IRc 1045 and IRc 1050 isolated from L. leucocephala grown in Nigeria. The persistence of inoculated effective Rhizobium strains after inoculation is desirable since it removes the need for reinoculation. Because of the perennial nature of L. leucocephala and its use in long-term alley farming experiments, we examined the persistence of inoculated rhizobial strains after inoculation, and their ability to sustain N2-fixation and biomass production at Ibadan. In 1992, ten years after Rhizobium introduction, uninoculated, L. leucocephala fixed about 150 kg N ha-1 yr-1 or about 41% of total plant N compared to 180 kg N ha-1 yr-1 or 43% measured in 1982. Serological typing of the nodules using the Enzyme-Linked-Immunosorbent Assay (ELISA) and intrinsic resistance to the streptomycin test revealed that most of the nodules (96%) formed on L. leucocephala in 1992 were by Rhizobium strains IRc 1045 and IRc 1050, which were inoculated in 1982. Nodules were absent on uninoculated L. leucocephala grown on the adjacent field with no history of L. leucocephala cultivation. We conclude that the N2 fixed by Rhizobium strains IRc 1045 and IRc 1050 persisted for many years in the absence of L. leucocephala and sustained effectively fixed N2 which growth and yield of L. leucocephala after several years, thus encouraging a possible low-input alley farming system by smallholder farmers in Nigeria.
    Type of Medium: Electronic Resource
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