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Diagnosis of bronchopulmonary infections by quantification of microflora (1994)

Pirali, F. ; Longo, M. ; Gelmi, M. ; [et al.]

Springer

European journal of epidemiology 10 (1994), S. 703-706

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ISSN: 1573-7284

Keywords: Bronchial microflora ; Bronchopulmonary infections ; Quantification

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract The quantification of bacteria and fungi in sputum or bronchoaspirate is of clinical value for the diagnosis of respiratory tract infections. We have developed an easy method to count the micro-organisms in patients with respiratory tract infections. This consists of the quantification of micro-organisms by subsequent streakings of a calibrated loop on agar. The correlation between microbiological quantitative data and the clinical status of patients with lower respiratory tract infections is discussed. The data seem to indicate that certain bacteria present in sputum or bronchoaspirate above a certain concentration may be responsible for lower respiratory tract infections. In patients with immunological disorders or chronic pathologies even lower concentrations of micro-organisms in bronchial secretions probably are enough to cause infections. The advantage of this counting method of the microbic species from the respiratory tract consists of their quantification: thus we can attribute an etiological role to a high concentration of the germs, while micro-organisms at low concentrations are probably contaminants. By this method isolated colonies are obtained after 12–18 hours. The bacterial quantification, by respiratory samples examination of the same patient in the following days, allows us to evaluate the efficacy of antibacterial therapy, producing a reduction of bacterial concentration.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01719285

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Ligninolytic enzymes from corncob cultures of Phanerochaete chrysosporium under semi-solid-state conditions (1999)

Couto, S. Rodríguez ; Longo, M. A. ; Cameselle, C. ; [et al.]

Berlin : Wiley-Blackwell

Acta Biotechnologica 19 (1999), S. 17-25

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ISSN: 0138-4988

Keywords: Life Sciences ; Life Sciences (general)

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: The effects of adding some inducers of lignolytic activity to semi-solid-state cultures of Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) were investigated. The inducers assayed were veratryl alcohol and solid manganese (IV) oxide. The microorganism was cultured on corncob, which functioned both as physical support and source of nutrients.Supplementing the cultures with veratryl alcohol created the situation where manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) activities of approximately 1,500 U/l and 200 U/l, respectively, could be attained. These activities were considerably higher than those obtained in the reference cultures (about 5 and 4-fold).In the same way, the addition of manganese (IV) oxide led to MnP and LiP activity levels of about 2,000 U/l and 300 U/l, respectively. These activities were also notably above (about 6 and 5-fold, respectively) those achieved in the reference cultures.Moreover, laccase activity (around 200 U/l) was only detected in veratryl alcohol or manganese (IV) oxide supplemented cultures.

Additional Material: 1 Ill.