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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 15 (1996), S. 31-33 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In this work, the citric acid production in solid state culture was performed, evaluating the isolated effect and interactions of particle size and liquid phase employed, by means of the factorial design of first order. The results indicate that the particle size is the most determinant variable. An analysis comparing submerged and solid state in optimal conditions was performed. When solid state culture was used, the productivity of citric acid was doubled, reducing the fermentation time from 14 to 6 days, compared to the submerged culture, obtaining a maximum citric acid concentration of 21.24 g/l.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Enzyme and Microbial Technology 16 (1994), S. 72-78 
    ISSN: 0141-0229
    Keywords: Pichia stipitis ; Xylose ; cell immobilization ; pulsing bioreactor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim : To study the efficacy of a 7-day quadruple regimen combining pantoprazole, bismuth, tetracycline and metronidazole as rescue treatment for Helicobacter pylori infection after failure of standard triple therapy.Methods : A prospective study was made of 140 patients infected with H. pylori and diagnosed with peptic ulcer or non-ulcer dyspepsia in whom triple therapy with proton pump inhibitor, clarithromycin and amoxicillin had failed. The patients were treated with quadruple therapy including pantoprazole, 40 mg twice daily, colloidal bismuth subcitrate, 120 mg four times daily, tetracycline, 500 mg four times daily, and metronidazole, 500 mg three times daily, for 7 days. Two months after completion of therapy, a 13C-urea breath test was performed to confirm eradication.Results : With quadruple therapy, the H. pylori eradication rates were 82% (95% confidence interval (CI), 75–88%) by ‘intention-to-treat’ and 85% (95% CI, 79–91%) by ‘per protocol’. No major side-effects were observed. No differences in eradication success were observed in relation to underlying disease (peptic ulcer: 85% (95% CI, 76–91%) vs. non-ulcer dyspepsia: 83% (95% CI, 68–93%)) or smoking habits (smokers: 86% (95% CI, 75–93%) vs. non-smokers: 83% (95% CI, 71–91%)).Conclusion : Quadruple therapy with pantoprazole, bismuth, tetracycline and metronidazole for 7 days is an effective H. pylori eradication treatment for patients in whom standard triple therapy has failed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 20 (2004), S. 0 
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background : The efficacy of azathioprine in the management of steroid-dependent ulcerative colitis is taken for granted. However, study populations frequently include together steroid-dependent and refractory patients.Aim : To assess the efficacy and safety of thiopurinic immunomodulators in strictly defined steroid-dependent ulcerative colitis.Methods : Survey of 34 patients with steroid-dependent ulcerative colitis, treated with azathioprine according to protocol. Therapeutical success: glucocorticoid withdrawal within 12 months, without steroid requirements during another year.Results : Mean age was 39.1 ± 17 years. Pancolitis and extensive colitis accounted for 50% of cases. Therapeutic success of immunomodulator treatment reached 70.6%, intention to treat analysis (confidence interval 95%: 52–84%) and 72.7%, as per protocol (confidence interval 95%: 54–86%). Mean time to steroid withdrawal was 4.6 months. In therapy successes, mean corpuscular volume and total serum bilirubin increased with treatment time (P = 0.0001). Fifteen adverse effects were observed in 13 patients (38%). Azathioprine was withdrawn in seven cases (20.6%); in four of them (with liver toxicity), treatment with mercaptopurine was indicated.Conclusions : Therapy with thiopurinic immunomodulators (azathioprine) represents the first option in the management of steroid-dependent ulcerative colitis. Its efficacy (70%) and its acceptable safety support this view. Increasing mean corpuscular volume and serum bilirubin values may be a surrogate marker of a beneficial effect.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 10 (1994), S. 75-81 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract When the immobilized cells are employed in packed-bed bioreactors several problems appear. To overcome these drawbacks, a new bioreactor based on the use of pulsed systems was developed [1]. In this work, we study the glucose fermentation by immobilized Saccharomyces cerevisiae in a packed-bed bioreactor. A comparative study was then carried out for continuous fermentation in two packed-bed bioreactors, one of them with pulsed flow. The determination of the axial dispersion coefficients indicates that by introducing the pulsation, the hydraulic behaviour is closer to the plug flow model. In both cases, the residence time tested varied from 0.8 to 2.6 h. A higher ethanol concentration and productivity (increases up to 16%) were achieved with the pulsated reactors. The volumes occupied by the CO2 were 5.22% and 9.45% for fermentation with/without pulsation respectively. An activity test of the particles from the different sections revealed that the concentration and viability of bioparticles from the two bioreactors are similar. From the results we conclude that the improvements of the process are attributable to a mechanical effect rather than to physiological changes of microorganisms.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 10 (1994), S. 61-73 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract This paper describes a new pulsing device which permits the insertion in pulsing form of a liquid phase fed into an equipment where a microbial or enzymatic transformation occurs. It also analyzes the modifications of the flow model caused by the pulsation generated by means of three kinds of pulsators: A hydropneumatic pulsator, a selfpropelled pulsator and a newly designed elastic membrane pulsator. The hydrodynamic behaviour of a packed-bed column, to which each of these pulsators has been connected is compared with the correspondent system without pulsation. The flow model is determined by the study of the curves of residence times distribution, obtained by using a stimulus-response technique. A computer programme has been used to determine the axial dispersion coefficients from the response curves. In all cases we worked within a wide range of Re p(10–215). The pneumatic pulsing device causes a backmixing in the system due to the alternative movement that communicates to the fluid contained in the column. The application of the pulsation by means of the selfpropelled injector or the elastic membrane pulsator reduces the axial dispersion coefficient in regard to those corresponding to the system without pulsation, a fact which becomes even more significant when operating at high flow velocities. For the study of the hydraulic model of the pulsing system of elastic membrane we worked under different conditions in order to determine the effect of the particle's diameter, the viscosity, and the frequency of pulsation. At the same frequency at which the Re pincreases, the axial dispersion coefficient also increases, following in all cases an almost linear tendency. For the same Re p, the dispersion coefficient decreases when the particle diameter increases. On the other hand, the dispersion coefficients obtained for a fluid of a viscosity of 2 cp are, in some cases, up to 3 times higher than the corresponding values for water. All the results indicate that the new pulsator is specially applicable to reactors which, as in many biological transformations, present inhibitory problems by the product, or, in general, to those reactions that require that the flow model in the reactor be a plug flow model.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Biotechnology letters 19 (1997), S. 995-998 
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Cultures of Phanerochaete chrysosporium, operating with an inert carrier (nylon sponge) and a non-inert carrier (barley straw), were employed in order to study laccase production during semi-solid state conditions. Manganese (IV) oxide, added to the cultures increased laccase activity 16-fold especially in barley straw cultures, in which a maximum laccase activity of 360 U/l (one unit is defined as 1 μmol of 2,2‘-azino-di-[3-ethyl-benzothiazoline-(6)-sulphonicacid] oxidized per minute) was achieved.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Biotechnology techniques 10 (1996), S. 53-58 
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Citric acid production by immobilized of Aspergillus niger in a fluidized bed reactor was performed, evaluating the productivity and the stability of the process when pulsing device was used. The application of a pulsing flow to fluidized bed reactor and the feed nitrogen limited allow to control of bioparticles morphology avoiding bed compactation. When operated at optimum pulsation frequency (0.3 s−1) the stability of the bioreactor was maintained for more than 30 days, increasing the citric acid production in more than 52.2%.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Aspergillus niger was immobilized by adsorption and entrapment in polyurethane foams and the efficiency of retention capacity, citric acid productivity and the operational stability of a fluidized bed reactor were then compared. The adsorption technique was superior to the entrapment technique, and it was possible to obtain bioparticles capable of keeping their activity for more than 25 days.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Biotechnology letters 22 (2000), S. 1443-1447 
    ISSN: 1573-6776
    Keywords: immersion bioreactor ; LiP ; MnP ; Phanerochaete chrysosporium ; solid state fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The production of manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) by the fungus Phanerochaete chrysosporium (ATCC 24725) in a new bioreactor, the Immersion Bioreactor, which grows cells under solid-state conditions, was studied. Maximum MnP and LiP activities were 987 U l−1 and 356 U l−1, respectively. The polymeric dye, Poly R-478, was degraded at 2.4 mg l−1 min−1 using the extracellular culture filtrate.
    Type of Medium: Electronic Resource
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