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Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts (1987)

Akamatsu, T. ; Sekiguchi, J.

Springer

Archives of microbiology 146 (1987), S. 353-357

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ISSN: 1432-072X

Keywords: Protoplast ; Plasmid ; Transformation ; Bacillus subtilis ; Linkage

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Competent cells of Bacillus subtilis were transformed with DNA from gently lysed protoplasts. Significant linkages among markers separated by distances of approximately 2.3% of the total chromosome were found, which have not been detected for conventional transformation. In comparison to previous reports, enhanced plasmid transformation was observed [4.0×107 transformants per μg DNA (one transformant per 5×104 molecules added)], when competent cells were transformed with DNA from lysed protoplasts harboring pUB110.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00410935

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